Thermo Fisher(Life)MitoTracker? Orange CMTMRos 橙色
目錄號(hào) | M7510 | 售價(jià) | 450.00元 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
規(guī)格 | 50 μg | 運(yùn)輸溫度 | 冰袋運(yùn)輸。 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
名稱 | MitoTracker? Orange CMTMRos 橙色 | 保存溫度 | -20℃避光干燥保存,2年有效 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
CAS號(hào) | N/A | 有效期 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
應(yīng)用 | 訂購(gòu)數(shù)量 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
產(chǎn)品簡(jiǎn)介: MitoTracker? Orange CMTMRos 橙色線粒體熒光探針 訂購(gòu)信息:
以上所有產(chǎn)品均為L(zhǎng)ife(Invitrogen)原裝正品,原始包裝為20x50 μg(20支50μg單個(gè)包裝構(gòu)成)。我司對(duì)M7510進(jìn)行拆開銷售,特惠價(jià)為450元/支,單支50μg,現(xiàn)貨銷售,歡迎訂購(gòu)。
基本介紹: MitoTracker? Orange CMTMRos(M7510)是一種橙色熒光染料,用于對(duì)活細(xì)胞線粒體進(jìn)行染色,該染料的積累取決于膜電位。乙醛固定后,該染料可穩(wěn)定保存。
產(chǎn)品特性 1) CAS NO.:N/A 2) 分子式:C24H24Cl2N2O 3) 分子量:427.37 4) 外觀:紫色固體(purple solid) 5) 激發(fā)/發(fā)射:554/576nm 6) 結(jié)構(gòu)式:
7) 溶解性:DMSO(1mM) 染色示例 Fig 1.A549 spheroid mounted with Prolong Glass and imaged with confocal microscope with lateral and axial projections.A549 spheroid grown in Nunclon Sphera microplates (Cat. No. 174925) at 1000 cells/well. Live cells were labeled with NucBlue Live ReadyProbes Reagent (Cat. No. R37605), MitoTracker Orange (Cat. No. M7510), and CellROX Deep Red Reagent for oxidative stress detection (Cat. No. C10422) for 1 hour at 37C. Cells were then fixed with 4% PFA, transferred to a #1.5 coverslip, and mounted in ProLong Glass. Sample was allowed to cure overnight exposed to air, and the coverslip was mounted to the slide using 100% glycerol. Image acquisition and analysis was performed using a Zeiss LSM710 Confocal running Zen software. MitoTracker Probes系列探針專題: 1) 傳統(tǒng)線粒體探針的缺點(diǎn): 傳統(tǒng)線粒體探針如羅丹明123(Rhodamine 123)雖然可立即聚集在功能線粒體上,然而,一旦線粒體膜電位喪失,這些探針就能很容易清洗掉離開細(xì)胞。當(dāng)碰到細(xì)胞需要進(jìn)行醛類固定或細(xì)胞內(nèi)含影響膜電位藥物的實(shí)驗(yàn)中,這些探針則受到限制,不可使用。 2) MitoTracker線粒體探針的優(yōu)點(diǎn) Mitotracker正克服傳統(tǒng)探針的缺點(diǎn)而被生產(chǎn),其能夠聚集在活線粒體上,并且細(xì)胞固定中也能良好維持。其中,Mitotracker orange,red,and deep red這三種探針經(jīng)透化(permeabilization)后仍能良好維持。在接下來免疫細(xì)胞化學(xué)(ICC)、原位雜交(ISH)或電鏡實(shí)驗(yàn)的處理步驟中樣本仍保留線粒體的活細(xì)胞染色形態(tài)。另外,Mitotracker探針去除病原細(xì)胞線粒體染色可能遇到的一些問題,因?yàn)橐坏┚€粒體染色后,細(xì)胞即可被固定然后進(jìn)行結(jié)果分析。 3) MitoTracker線粒體探針產(chǎn)品 我們提供7種Mitotracker探針,它們?cè)诠庾V特性、氧化態(tài)以及可否固定幾個(gè)參數(shù)上有所差異,具體見下表。
【注1】:MitoTracker? Green FM (#M7514)在水溶液中無熒光; 【注2】:MitoTracker? Orange CM-H2TMRos(#M7511),MitoTracker? Red CM-H2XRos(#M7513)為還原態(tài)探針,無熒光。只有被氧化后才發(fā)熒光。 |