德國(guó)MN Nucleobond Xtra Midi Plus 超快速轉(zhuǎn)染級(jí)質(zhì)粒中提試劑盒
貨號(hào):740412.50
規(guī)格:50 preps
品牌:德國(guó)MN
從大腸桿菌中提取高拷貝/低拷貝質(zhì)粒。新一代陰離子交換柱-短時(shí)間,高產(chǎn)量,轉(zhuǎn)染用質(zhì)粒。
特點(diǎn):
節(jié)約60%的時(shí)間,增加100%的產(chǎn)量
新一代陰離子交換–超快,高產(chǎn)量的轉(zhuǎn)染級(jí)別的質(zhì)粒DNA
優(yōu)化設(shè)計(jì)的柱子:制備時(shí)間約為30 分鐘(Midi prep);35 分鐘(Maxi prep)
包含柱過濾器:高過慮流速,清除裂解液中不溶物的同時(shí)可向柱子加樣
改進(jìn)的硅材料:標(biāo)準(zhǔn)的產(chǎn)量250μg-1000μg
專li的陰離子交換技術(shù)得到轉(zhuǎn)染級(jí)別的質(zhì)粒DNA
NucleoBond(R)Xtra Plus kits 包含NucleoBond(R) Finalizers;加速了DNA的沉淀
2nd generation of anion exchanger for fast purification of plasmid DNA, NucleoBond Finalizer included
Application | Transfection-grade plasmid DNA isolation |
Selling unit | 10 Prep(s), 50 Prep(s) |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Midi prep |
Handling | Gravity flow |
Lysate clarification | Column filter |
Automated use | No |
Sample material | Bacteria, E. coli |
Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
Vector size | 2?50 kbp |
Typical yield | 500 μg |
Theoretical binding capacity | 1000 μg |
Typical purity A260/A280 | 1.8?1.95 |
Endotoxin level | 1?10 EU/μg DNA |
Isopropanol precipitation | NucleoBond Finalizer |
Preparation time | 30 min/prep |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 39 Month(s) |
Hazardous material | Yes |