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Lagerung bei +4 \241)-31(C bis \32020 \241)-31(C je)]TJ 0 -1.08334 TD [(nach Bedar)-21(f!)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 1008.0401 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(olymerisationsl\232sung )]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 1000.1228 Tm 0 Tr 0 0 0 1 k 0.0001 Tc (D)Tj 0.68811 -0.00002 TD 0 Tc 0 Tw (ie gek\237hlten Stamml\232sungen A und B sind unmittelbar vor)Tj -0.68811 -1.08332 TD [(G)-10(ebrauch im V)52(erh\212ltnis 9 T)104(eile \(v/v\) Stamml\232sung A \()-21(Mess-)]TJ T* [(z)-16(ylinder)-21(\) und 1 T)104(eil Stamml\232sung B \()-21(Pipette\) in einem Becher-)]TJ 0 -1.08334 TD [(glas mit Hilfe eines G)-10(lasstabes zu mischen.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 971.2883 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(olymerisation)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 963.3708 Tm 0 Tr 0 0 0 1 k -0.003 Tc 0 Tw [(Die P)21(roben werden anschlie\247end bei orientier)-21(ter P)21(osition lang-)]TJ 0 -1.08334 TD [(sam mit dem P)21(olymerisationsgemisch randvoll \237bergossen und)]TJ 0 -1.08333 TD [(anschlie\247end leicht evakuier)-21(t. Die Evakuierung entweder im vor)1(-)]TJ T* [(gek\237hlten Exsikkator bei 4 \241)-31(C \()-21(leichtes V)63(akuum, z.)-169(B)-21(. W)31(asser-)]TJ 0 -1.08334 TD [(strahlpumpe bei 200 mbar)-21(\) oder bereits im T)11(iefk\237hlschrank mit)]TJ 0 -1.08333 TD [(ex)-10(tern angeschlossener V)63(akuumpumpe f\237r ca. 1)105(0 Minuten)]TJ T* [(durchf\237hren. Die P)21(olymerisation er)-20(folgt)-1( im Bereich von \3202 bis )]TJ T* [-31(C und sollte in 24 Stunden abgeschlossen sein. Die P)21(o)-1(l)-1(y-)]TJ 0 -1.08334 TD [(merisationszeiten sind abh\212ngig vom P)21(olymerisationsvolumen)]TJ 0 -1.08333 TD [(sowie der T)104(emperatur)42(. G)-10(r\232\247ere P)21(roben sind somit bei niedrige)1(-)]TJ -0.0026 Tc (r)' 0.33039 -0.00002 TD -0.003 Tc [(en T)104(emperaturen auszuh\212r)-20(ten. D)-1(abei ist die K\212ltekapazit\212t der)]TJ -0.33039 -1.08332 TD [(ver)-21(wendeten K)10(\237hleinrichtung \()-20(T)9(iefk\237hlfach im Eisschrank, T)10(ief-)]TJ 0 -1.08333 TD [(k\237hltruhe, T)10(iefk\237hlschrank, T)11(iefk\237hlwanne z.)-169(B)-21(. f\237r P)21(araffinbl\232cke)]TJ 0 -1.08334 TD [(mit Deckelverschluss)-21(\) zu beachten. Reproduzierbare Ergebnisse)]TJ 0 -1.08333 TD [(f\237r verschiedene P)21(robengr\232\247en werden in einem exgesch\237tz)-16(ten)]TJ T* [(T)9(iefk\237hlschrank mit variabel einstellbaren T)105(emperaturen z)-16(w)-1(i)-1(-)]TJ 0 -1.08334 TD [(schen)-232(\3202 bis)-233(\32025)-232-32(C bei einer)-176(T)103(emperaturkonstanz von +/)112(\3200,5)-232-31(C)]TJ 0 -1.08333 TD [(erzielt. W)31(\212hrend der P)21(olymerisation die G)-10(ef\212\247e nicht \232ffnen.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 843.5362 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(W)31(eitere V)63(erarbeitung)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 835.6189 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Haben sich die P)Tj 7.49951 0.00001 TD [(roben nach der P)21(olymerisation auf Raumtem-)]TJ -7.49951 -1.08334 TD [(peratur er)-21(w\212rmt, kann mit Histobl\232cken und T)104(echnovit 3040)]TJ T* [(aufgeblock)-16(t werden, um die P)21(roben aus der T)104(eflon F)21(orm zu ent-)]TJ 0 -1.08333 TD (nehmen.)Tj 30.23623 42.88449 TD [(Im Anschluss folgt das S)-10(chneiden der P)21(roben, wobei w\212hrend)]TJ 0 -1.08334 TD [(des S)-10(chneidens der Block mit 30)-169(%-igem Ethanol zu befeuchten)]TJ 0 -1.08332 TD [(ist. Die S)-10(chnitte werden nun abgenommen und auf beschichte-)]TJ 0 -1.08334 TD [(ten Objek)-16(ttr\212gern mit 96)-169(%-igem Ethanol gestreck)-16(t. S)-10(chlie\247lich)]TJ T* [(werden die S)-10(chnitte mit P)-21(E)-10(-)-10(F)21(olie abgedeck)-16(t \237ber Nacht bei )]TJ 0 -1.08332 TD [(3)41(7 )1-32(C bi)1(s )-1(60 )1-57(C gepresst und vor dem F)21(\212rben entplastet.)]TJ 0 -1.08334 TD (Ausf\237hrliche Informationen zur Fixation, Dehydration,)Tj T* (Entplastung und Routinef\212rbungen finden Sie in unserem)Tj 0 -1.08332 TD [(P)21(rospek)-16(t oder unter www)42(.kulzer-technik.de)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 1012.0912 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(V)63(erarbeitungshilfen f\237r T)104(echnovit 9)73(1)94(0)-1(0)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 1004.1739 Tm 0 Tr 0 0 0 1 k 0.0001 Tc Tj 0.98011 0 Td 0 Tc 0 Tw [(Einbettform aus T)104(eflon)]TJ -0.98011 -1.08332 TD 0.0001 Tc Tj 0.98012 0 Td 0 Tc (Histobl\232cke)Tj -0.98012 -1.08334 TD -0.0005 Tc Tj 0.979 0 Td 0 Tc [(P)20(olyethylen)1(f)-1(o)1(lien)]TJ -0.979 -1.08334 TD -0.0001 Tc Tj 0.97983 0 Td 0 Tc [(Objek)-16(ttr\212gerpresse)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 975.3392 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Gefahrenhinweise/)-21(Sicherheitsratschl\212ge)]TJ 0 -1.31954 TD [(Bitte beachten Sie die Hinweise auf den P)21(roduktverpackun-)]TJ 0 -1.08333 TD [(gen und Sicherheitsdatenbl\212ttern)-21(. )]TJ 0 -1.08334 TD [(www)42(.kulzer-technik)-21(.de )]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 947.9219 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Unsere anwendungstechnische Beratung in W)31(o)-1(r)-21(t, S)-10(chrift und)]TJ 0 -1.08333 TD 0.0002 Tc (d)Tj 0.58321 -0.00002 TD 0 Tc [(urch V)52(ersuche er)-21(folgt nach bestem Wissen, gilt jedoch nur als)]TJ -0.58321 -1.08332 TD [(unverbindlicher Hinweis, auch in Bezug auf etwaige S)-10(chutz-)]TJ 0 -1.08333 TD [(rechte Dritter)42(, und befreit Sie nicht von der eigenen P)21(r\237fung der)]TJ 0.0002 Tc (v)' 0.45815 -0.00002 TD 0 Tc [(on uns geliefer)-21(ten P)21(roduk)-16(te auf ihre Eignung f\237r die beabsich-)]TJ -0.45815 -1.08332 TD [(tigten V)52(er)-21(fahren und Zwecke. Anwendung, V)52(er)-21(wendung und)]TJ 0 -1.08333 TD [(V)52(erarbeitung der P)21(roduk)-16(te er)-21(folgen au\247erhalb unserer K)16(ontroll-)]TJ 0.0001 Tc (m)' 0.85413 -0.00002 TD 0 Tc [(\232glichkeiten und liegen daher ausschlie\247lich in Ihrem V)52(erant-)]TJ -0.85413 -1.08332 TD [(w)-1(o)-1(r)-21(tungsbereich. S)-10(elbstverst\212ndlich gew\212hrleisten wir die ein-)]TJ 0 -1.08333 TD [(wandfreie Qualit\212t unserer P)21(roduk)-16(te nach Ma\247gabe unserer)]TJ T* [(Allgemeinen V)52(erkaufs- und Lieferbedingungen.)]TJ 0 -2.6391 TD [(*)-813(Detaillier)-21(te Informationen \237ber unsere P)21(roduk)-15(te finden Sie )]TJ 1.16667 -1.08334 TD [(unter www)42(.kulzer-technik.de)]TJ -1.16667 -2.63912 TD [-348(= eingetragenes W)31(arenzeichen, K)10(ulzer GmbH)-21(, Hanau)]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 195.5904 1079.5267 m 195.5904 686.9277 l S 195.5907 671.6163 m 195.5907 276.3763 l S 195.5904 259.5777 m 195.5904 15.5907 l S 377.0078 1079.5259 m 377.0078 816.3779 l S 377.0078 671.1008 m 377.0078 403.1208 l S 377.0077 259.578 m 377.0077 15.591 l S 20.268 951.133 m 189.496 951.133 l S BT /F1 1 Tf 6 0 0 6 23.1022 953.4716 Tm [(Bezeichnung)-8896(Menge)-4538(Materialnr)42(.)]TJ 0 -1.55577 TD [(T)104(echnovit 9)83(1)103(00 Basisl\232sung)-2540(1)103(.)-1(0)-1(00)-170(g)-4474(1)]TJ 0 -1.08333 TD [(stabilisier)-21(t)]TJ 0 -1.31955 TD [(T)104(echnovit 9)83(1)103(00 PM)-42(MA-)-10(P)22(ulver)-1719(1)104(2)-1(0)-169(g)-5245(2)]TJ 0 -1.31957 TD [(T)104(echnovit H\212r)-21(ter 1)-6604(8 Beutel \207)-236(1)-57(g)-1684(3)]TJ 0 -1.31955 TD [(T)104(echnovit H\212r)-21(ter 2)-6604(1)103(0 ml)-5183(4)]TJ T* [(T)104(echnovit 9)83(1)103(0)-1(0)-8145(5 ml)-5642(5)]TJ 0 -1.08333 TD [(P)21(olymerisations-)-10(Regler)]TJ ET 20.055 896.195 169.654 64.063 re s 107.7164 960.4718 m 107.7164 895.9827 l S 153.0707 960.471 m 153.0707 895.983 l S BT /F1 1 Tf 6 0 0 6 286.7241 788.6147 Tm [(Basis)-3767(P)22(u)-1(lver)-3433(H\212r)-21(ter 1)-2538(H\212r)-20(ter 2)-2538(Regler)-3309(Lagerung/)-41(Haltbarkeit der)]TJ ET 201.685 779.776 m 552.331 779.776 l S BT /F1 1 Tf 6 0 0 6 470.9761 782.1147 Tm (L\232sungen bei:)Tj ET 201.685 770.442 m 552.331 770.442 l S BT /F1 1 Tf 6 0 0 6 204.5195 772.78 Tm [(K)16(omponentennummer)-3779(1)-5580(2)-5579(3)-5580(4)-5580(5)]TJ ET 201.685 761.107 m 552.331 761.107 l S BT /F1 1 Tf 6 0 0 6 204.5195 763.4454 Tm [(P)21(r\212infiltration 3)-7139(200 ml)-9241(1)-169(g)-17132-31(C bis zu )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 509.2891 763.4454 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 516.2896 763.4454 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Jahr)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 751.772 m 552.331 751.772 l S BT /F1 1 Tf 6 0 0 6 204.5195 754.1108 Tm [(Infiltration)-9451(ad 250 ml)-1704(20 g)-4162(1)-169(g)-169(/)-169(*)-1(*)-1(2)-169(g)-14438-31(C bis zu )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 509.2891 754.1108 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 516.2896 754.1108 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Jahr)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 742.438 m 552.331 742.438 l S BT /F1 1 Tf 6 0 0 6 204.5195 744.7761 Tm (Stamml\232sung A)Tj 13.70077 0.00001 TD [(ad 500 ml)-1704(80 g)-4162(3)-169(g)-170(/)-169(*)-1(*4)-169(g)-14438-31(C bis zu )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 509.2891 744.7761 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 516.2896 744.7761 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Jahr)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 733.103 m 552.331 733.103 l S BT /F1 1 Tf 6 0 0 6 204.5195 735.4415 Tm [(Stamml\232sung B)-6638(ad 50 ml)-14550(4 ml)-4225(2 ml)-4224-32(C bis zu )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 509.2891 735.4415 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 516.2896 735.4415 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Jahr)Tj -51.96169 -1.55578 TD [(P)21(olymerisatonsgemisch)-3326(9 V)52(olumenteile)-14832(Stamml\232sung A)]TJ 25.98424 -1.08332 TD [(u)-1(n)-1(d)-16717(S)-16(ofor)-21(t ver)-21(wenden)]TJ -12.28347 -1.08334 TD 0.0003 Tc (1)Tj 0.8545 0 Td 0 Tc [(V)52(olumenteile)-14832(Stamml\232sung B)]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.472 710.787 351.071 84.6141 re s 283.4643 795.6138 m 283.4643 710.5749 l S 320.3152 795.6138 m 320.3152 733.2518 l S 357.1655 795.6142 m 357.1655 733.2522 l S 394.0159 795.6136 m 394.0159 733.2516 l S 430.8663 795.614 m 430.8663 733.252 l S 467.7163 795.6138 m 467.7163 710.5748 l S Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 701.345 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Achtung!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 693.4277 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(**Nur bei V)52(er)-21(wendung von stabilisier)-21(ter T)104(echnovit 9)84(1)103(00 Basisl\232sung werden 2 g \()-21(Infiltrationsl\232sung)-21(\) und 4 g \(Stamml\232sung)1( A)-21(\) der)]TJ 0 -1.08334 TD (Basisl\232sung zugegeben.)Tj ET 0 J 0 j 10 M []0 d 1 w 0 0 0 1 K 19.843 678.189 m 552.756 678.189 l S 19.843 267.874 m 552.756 267.874 l S BT /F1 1 Tf 6 0 0 6 201.2598 666.1611 Tm 0.0001 Tc (m)Tj 0.8541 -0.00001 TD 0 Tc [(agnetic stirrer until the powder is completely dissolved. T)10(he)]TJ -0.85409 -1.08333 TD (solution has to be clear)Tj 10.18701 0.00001 TD 0.0001 Tc (.)Tj 0.50022 0 Td 0 Tc (Hardener 1 \(3 g or 4 g)Tj 9.83328 0.00001 TD (\))Tj 0.54207 -0.00001 TD (is then added)Tj -21.06259 -1.08334 TD [(under fur)-21(ther stirring. Finally)42(, Basis solution is added until the)]TJ 0 -1.08333 TD 0.0001 Tc (5)Tj 0.56209 -0.00001 TD (00)Tj 1.41626 0 Td 0 Tc (ml graduation mark has been reached. )Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 637.3263 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(reparation of Stock S)-10(olution B)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 629.409 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (30 ml Basis solution is added to a 50 ml graduated measuring)Tj 0 -1.08333 TD (cylinder containing 4 ml of Hardener 2. Briefly mix, using a )Tj T* [(magnetic stirrer)42(. 2 ml Regulator is then added, followed by Basis)]TJ T* (solution until the 500 ml graduation mark has been reached. Stir)Tj T* (again using magnetic stirrer)Tj 12.12427 0.00001 TD -0.0002 Tc (.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 594.0743 Tm 0 Tr 0 0 0 1 k -0.006 Tc 0 Tw [(Attention: Hardener 2)-175(+)-176(Regulator must be dosed using a )]TJ 0 -1.08333 TD (pipette!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 579.657 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Store the solutions in stoppered brown glass b)Tj 20.51001 0.00001 TD (ottles, label )Tj -20.51001 -1.08335 TD [(accordingly and store at between +4 \241)-31(C and \32020 \241)-31(C as required.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 563.8224 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(olymerisation solution )]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 555.905 Tm 0 Tr 0 0 0 1 k 0.0001 Tc (M)Tj 0.85405 -0.00001 TD 0 Tc 0 Tw (ix the cooled stock solutions A and B in a glass beaker imme)Tj 26.7502 0.00002 TD (-)Tj -27.60425 -1.08334 TD [(diately before use, using a glass stirrer)42(, in a ratio of 9 par)-21(ts (v/v))]TJ T* [(stock solution A \()-21(graduated measuring cylinder)-21(\) and 1 par)-21(t)]TJ 0 -1.08333 TD [(stock solution B \()-21(pipette\).)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 527.0704 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62477 0.00001 TD 0 Tw (olymerisation)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 519.1531 Tm 0 Tr 0 0 0 1 k 0 Tc (T)Tj 0.5525 -0.00001 TD 0 Tw [(he polymerisation mix)-10(ture is poured slowly over the correctly)]TJ -0.5525 -1.08332 TD (orientated specimens up to the top, followed by slight )Tj 0 -1.08334 TD (evacuation.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 496.8184 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62477 0.00001 TD 0 Tw (re-cooled desiccator)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 488.9011 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Evacuate in the pre-cooled desiccator at 4 \241)-31(C \()-21(slight vacuum, for)]TJ 0 -1.08333 TD (example water jet pump at 200 mbar)Tj 16.25 0.00001 TD (\))Tj 0.54203 0 Td (or in the deep freezer with)Tj -16.79203 -1.08335 TD [(an ex)-10(ternal vacuum pump for ab)-10(out ten minutes.)]TJ 0 -1.31955 TD [(P)21(olymerisation between \3202 and \32020 \241)-31(C should be completed)]TJ 0 -1.08333 TD (within 24 hrs. P)Tj 6.77075 0.00001 TD (olymerisation times depend on polymerisation)Tj -6.77075 -1.08334 TD (volume as well as temperature. Larger specimens must be )Tj T* (hardened at lower temperatures. Cooling capacity of the cooling)Tj 0 -1.08333 TD [(appliance \()-21(freezer compar)-21(tment in the refrigerator)42(, large or small)]TJ T* (freezer)Tj 2.93677 0.00001 TD (,)Tj 0.50003 0 Td (freezer compar)Tj 6.66623 0.00001 TD (tment for example for paraffin block with)Tj -10.10303 -1.08336 TD [(lid)-21(\) must be considered. Reproducible results for a variety of)]TJ 0 -1.08333 TD (specimen sizes can be obtained in an explosion proof deep )Tj T* (freezer with variable temperature settings beween \3202 and )Tj 0 -1.08334 TD [-31(C at a constant temperature rate of +/\320 0.5 \241)-31(C. Do not)]TJ 0 -1.08333 TD (open the containers during polymerisation.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 11 0 0 11 59.5276 662.6288 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (User Instruction )Tj 0 -1.0952 TD [(T)104(echnovit 9)73(1)94(00)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 634.7469 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Application)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 626.8296 Tm 0 Tr 0 0 0 1 k 0 Tc (T)Tj 0.4585 -0.00001 TD 0 Tw [(echnovit 9)83(1)104(00 is a polymerisation system based on methyl met-)]TJ -0.4585 -1.08331 TD 0.0001 Tc (h)Tj 0.56208 -0.00001 TD 0 Tc (acr)Tj 1.39617 0.00002 TD (ylate which was specially developed for embedding minera)Tj 25.7483 0.00001 TD (-)Tj -27.70655 -1.08336 TD [(lized tissues for light microscopy)42(.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 604.4951 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(roper)-16(ties)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 596.5776 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Chemical polymerisation of the hydrophobe T)104(echnovit 9)83(1)104(00 is)]TJ 0 -1.08333 TD (per)Tj 1.45825 0.00001 TD (f)Tj 0.27103 -0.00001 TD [(ormed in the absence of oxygen with the help of a cataly)-21(tic)]TJ -1.72928 -1.08334 TD (system comprising a peroxide and an amine. Additional compo-)Tj 0 -1.08333 TD (nents such as PM)Tj 7.95898 0.00001 TD (MA powder and a regulator facilitate control)Tj 19.24903 0.00001 TD (-)Tj -27.20802 -1.08335 TD [(led polymerisation at low temperatures \()-21(from \3202 \241)-31(C to \32020 \241)-31(C )]TJ 0 -1.08334 TD [(depending on the amounts used)-21(\) and guarantee complete )]TJ 0 -1.08333 TD (dispersal of the heat generated by the polymerisation process.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 548.243 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Components of T)Tj 7.95802 0.00001 TD (echnovit 9)Tj 4.781 0.00001 TD -0.094 Tc [(10)-95(0)]TJ -12.73902 -14.08597 TD 0 Tc (Impor)Tj 2.74512 0.00001 TD (tant!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 455.8099 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Basis solution T)Tj 6.8545 0.00001 TD (echnovit 9)Tj 4.45848 0.00001 TD (1)Tj 0.4585 0.00001 TD (00 can be used in stabilized or desta-)Tj -11.77148 -1.08337 TD [(bilized form. F)21(or immunohistochemistr)-21(y analyses, the basis solu-)]TJ 0 -1.08333 TD (tion should be destabilized!)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 433.4753 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Destabilisation of the Basis S)Tj 13.67652 0.00001 TD (olution)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 425.558 Tm 0 Tr 0 0 0 1 k -0.005 Tc 0 Tw (Fill the chromatography column with 5)Tj 16.64597 0.00001 TD 0 Tc (0)Tj 0.72562 0.00001 TD -0.0055 Tc (g)Tj 0.84295 0 Td -0.006 Tc (of Al2O3 \()Tj 4.36057 0.00001 TD -0.0056 Tc (a)Tj 0.51538 -0.00001 TD -0.006 Tc (ctive, basic,)Tj -23.09049 -1.08336 TD -0.0055 Tc (90\))Tj 1.64398 0 Td -0.005 Tc [(a)1(n)1(d)1( )1(a)1(l)1(l)1(o)1(w)1( )1(t)1(h)1(e)1( )1(T)105(echnovit 9)83(1)105(00 basis solution \()-21(component no.)-64(1)104(\))]TJ -1.64398 -1.08333 TD -0.006 Tc [(t)-1(o)-1( )-1(f)-1(l)-1(o)-1(w)-1( )-1(t)-1(h)-1(r)-1(o)-1(u)-1(g)-1(h)-1( )-1(i)-1(t)-1( )-1(s)-1(l)-1(o)-1(w)-1(l)-1(y)41(. A column prepared as ab)-9(ove is sufficient)]TJ T* (to destabilize 3 \320 4 litres of basis solution. Store the destabilized)Tj 0 -1.08334 TD -0.005 Tc [(basis solution in por)-21(tions in stoppered brown glass b)-9(o)1(t)1(t)1(l)1(e)1(s)1( )1(a)1(t)1( )]TJ 0 -1.08333 TD -0.006 Tc [(either +4 \241)-31(C \()-21(max 5 days)-21(\) or at \3201)104(5)-1( )-1-32(C to \32020 \241)-31(C \()35(up to half a)]TJ T* [(year)-21(\).)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 377.2233 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(re-infiltration)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 369.306 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Mix 200 ml of the basis solution with 1g of Hardener 1 in a )Tj 0 -1.08333 TD [(200 ml glass container \()-21(using a glass spatula or magnetic stirrer)-21(\))]TJ T* (until the hardener is totally dissolved. P)Tj 17.04077 0.00001 TD (re-infiltrate the Specimen)Tj -17.04077 -1.08335 TD [(for approx 1 hour at RT)83(.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 340.4713 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Infiltration)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 332.554 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Mix 200 ml of the Basis mix)-10(ture with 1 to 2 g of Hardener 1 in a)]TJ 0 -1.08333 TD (200 ml glass container \()Tj 10.521 0.00001 TD [(use magnetic stirrer)-21(\), add 20)-169(g of pow-)]TJ -10.521 -1.08335 TD (der and continue to mix until the powder is completely dissol-)Tj 0 -1.08333 TD (ved. Infiltration of the specimen should be carried out at 4 )Tj 25.56177 0.00001 TD Tj 0.38525 0.00001 TD 0.0001 Tc (C)Tj 1.00025 0 Td 0 Tc (for)Tj -26.94727 -1.08335 TD 0.0001 Tc (1)Tj 0.85427 0 Td 0 Tc (to 24 hrs. F)Tj 4.95848 0.00001 TD (or large specimens, for example endoprostheses,)Tj -5.81275 -1.08335 TD (allow up to 48 hrs at low temperatures.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 290.7194 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(reparation of Stock S)-10(olution A:)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 282.8021 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (80 g powder are added stepwise to a 500 ml graduated measu-)Tj 0 -1.08333 TD (ring cylinder containing 400 ml of the Basis solution using a )Tj 0.54328 43.45086 TD [(Description)-9688(Amount)-4552(Material )]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 20.268 528.169 m 189.496 528.169 l S BT /F1 1 Tf 6 0 0 6 159.1651 530.5073 Tm (Number)Tj -22.67715 -1.55579 TD [(T)104(echnovit 9)83(1)103(00 Basis solution )-1457(1)103(.)-1(0)-1(00)-169(g)-4947(1)]TJ 0 -1.08333 TD (stabilized)Tj 0 -1.31955 TD [(T)104(echnovit 9)83(1)103(00 PM)-42(MA powder)-1207(1)104(2)-1(0)-169(g)-5717(2)]TJ T* [(T)104(echnovit Hardener 1)-5251(8 Sachet x)-179(1)-57(g)-2004(3)]TJ 0 -1.31954 TD (T)Tj 0.45848 0.00001 TD (echnovit 9)Tj 4.4585 0.00001 TD (1)Tj 0.4585 0.00001 TD (00 Hardener 2)Tj 9.27017 0.00001 TD (1)Tj 0.4585 0.00001 TD 0.0003 Tc (0)Tj 0.8545 0 Td 0 Tc (ml)Tj 6.7185 0.00001 TD (4)Tj -22.67715 -1.31962 TD [(T)104(echnovit 9)83(1)103(0)-1(0)-8145(5 ml)-6114(5)]TJ 0 -1.08334 TD (polymerisation regulator)Tj ET 20.055 474.062 169.654 69.732 re s 107.7165 544.0067 m 107.7165 473.8496 l S 155.9054 544.0066 m 155.9054 473.8496 l S BT /F1 1 Tf 6 0 0 6 286.7241 377.8026 Tm (Basis)Tj 6.14173 0.00001 TD (P)Tj 0.62477 0.00001 TD [(owder)-2788(Hardener 1)-1183(Hardener 2)-1184(Regulator)-1892(Storage/)-21(Shelf Life of the )]TJ ET 201.685 368.9641 m 552.331 368.9641 l S BT /F1 1 Tf 6 0 0 6 470.9761 371.3025 Tm [(S)-16(olution at:)]TJ ET 201.685 359.629 m 552.331 359.629 l S BT /F1 1 Tf 6 0 0 6 204.5194 361.968 Tm (Component Number)Tj 13.70078 0.00001 TD 5.58 Tc [(12)1(345)]TJ ET 201.685 350.295 m 552.331 350.295 l S BT /F1 1 Tf 6 0 0 6 204.5194 352.6333 Tm 0 Tc [(P)21(re \320 Infiltration 3)-5847(200 ml)-9241(1)-169(g)-17132-31(C up to )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 507.414 352.6333 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 514.4145 352.6333 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (year)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 340.96 m 552.331 340.96 l S BT /F1 1 Tf 6 0 0 6 204.5194 343.2986 Tm [(Infiltration)-9451(ad 250 ml)-1704(20 g)-4162(1)-169(g)-169(/)-169(*)-1(*)-1(2)-169(g)-14438-31(C up to )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 507.414 343.2986 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 514.4145 343.2986 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (year)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 331.6251 m 552.331 331.6251 l S BT /F1 1 Tf 6 0 0 6 204.5194 333.9641 Tm (Stock solution A)Tj 13.70078 0.00001 TD (ad 500 ml)Tj 6.14173 0.00001 TD (80 g)Tj 6.14173 0.00001 TD [(3)-169(g)-169(/)-169(*)-1(*)-1(4)-169(g)-14438-31(C up to )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 507.414 333.9641 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 514.4145 333.9641 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (year)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 322.291 m 552.331 322.291 l S BT /F1 1 Tf 6 0 0 6 204.5194 324.6293 Tm [(Stock solution B)-6492(ad 50 ml)-14550(4 ml)-4225(2 ml)-4225-31(C up to )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 507.414 324.6293 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 514.4145 324.6293 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (year)Tj -51.64919 -1.55576 TD (P)Tj 0.62477 0.00001 TD (olymerisation mix)Tj 7.61401 0.00001 TD [(ture)-3754(9 par)-21(ts (v/v))-16113(Stock S)-16(olution A)]TJ 17.74547 -1.08336 TD [(plus)-16592(Use immediately)]TJ -12.28347 -1.08333 TD 0.0001 Tc (1)Tj 0.85425 0 Td 0 Tc (par)Tj 1.45825 0.00001 TD 0.0001 Tc (t)Tj 0.5841 0 Td 0 Tc (\(v/v)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6.3 0 0 6 313.2246 302.2947 Tm 0 Tr 0 0 0 1 k 0 Tc (\))Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 414.2832 302.2947 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Stock S)Tj 3.43285 0.00001 TD (olution B)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.472 299.975 351.071 84.6141 re s 283.4644 384.8016 m 283.4644 299.7626 l S 320.3153 384.8016 m 320.3153 322.4396 l S 357.1656 384.802 m 357.1656 322.44 l S 394.016 384.8014 m 394.016 322.4394 l S 430.8665 384.8018 m 430.8665 322.4398 l S 467.7164 384.8016 m 467.7164 299.7626 l S Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 290.7454 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Impor)Tj 2.74512 0.00001 TD (tant!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 282.8281 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (**Only when using stabilized T)Tj 13.146 0.00001 TD (echnovit 9)Tj 4.45848 0.00001 TD (1)Tj 0.4585 0.00001 TD (00 basis solution, 2g infiltration solution and 4g \()Tj 21.22925 0.00001 TD [(stock solution A)-21(\) must be added to the ba-)]TJ -39.29224 -1.08338 TD (sis solution.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 11 0 0 11 59.5276 251.815 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Mode d\325emploi )Tj 0 -1.0952 TD [(T)104(echnovit 9)73(1)94(00)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 222.4331 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Domaines d\325application)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 214.5157 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(e)-1(ch)1(no)-1(v)1(it 9)83(1)103(00 est un syst\217me de polym\216risation \210 base de)]TJ 0 -1.08333 TD (m\216thacr)Tj 3.625 0.00001 TD (ylate de m\216thyle, qui a \216t\216 sp\216cifiquement mis au point)Tj -3.625 -1.08334 TD (pour l\325inclusion de tissus min\216ralis\216s en microscopie optique.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 192.1811 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(ropri\216t\216 du mat\216riau)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 184.2638 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(La polym\216risation chimique du T)104(echnovit 9)83(1)104(00 hydrophobe )]TJ 0 -1.08333 TD (s\325effectue sous exclusion d\325oxyg\217ne, \210 l\325aide d\325un syst\217me de)Tj T* (catalyseur en peroxyde et en amine. Des composants compl\216)Tj 26.99927 0.00001 TD (-)Tj -26.99927 -1.08335 TD [(mentaires tels que la poudre de PM)-42(MA \()-21(polym\216thacr)-21(ylate de)]TJ 0 -1.08333 TD [(m\216thyle\) et un r\216gulateur)42(, permettent une polym\216risation )]TJ T* (contr\231l\216e au froid \()Tj 8.27052 0.00001 TD [(dans la plage de \3202 \210 \32020 \241)-31(C, en fonction )]TJ -8.27052 -1.08334 TD (de la quantit\216\), qui garantit une \216vacuation compl\217te de la )Tj T* (chaleur de polym\216risation.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 129.4291 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Composants T)104(echnovit 9)73(1)94(0)-1(0)]TJ 0 -14.15551 TD (Attention !)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 36.5787 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(La solution de base T)104(echnovit 9)83(1)104(00 peut \220tre utilis\216e aussi bien)]TJ 0 -1.08332 TD (stabilis\216e que d\216stabilis\216e. P)Tj 12.51978 0.00002 TD (our les examens immuno-histochi)Tj 14.85425 0.00002 TD (-)Tj -27.374 -1.0834 TD (miques, d\216stabiliser la solution de base!)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 255.0961 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (D\216stabilisation de la solution de base)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 247.1787 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Remplir la colonne de chromatographie de 50 g Al2O3 \()-21(actif,)]TJ 0 -1.08333 TD (basique, 90\), et laisser la solution de base T)Tj 18.97876 0.00001 TD (echnovit 9)Tj 4.4585 0.00001 TD (1)Tj 0.45849 0.00001 TD 0.001 Tc (00)Tj -23.89575 -1.08336 TD 0 Tc (\()Tj 0.271 0.00001 TD (num\216ro de composant 1)Tj 10.771 0.00001 TD 0.0001 Tc (\))Tj 0.54213 0 Td 0 Tc (s\325\216couler lentement. Un remplissage)Tj -11.58412 -1.08336 TD (de la colonne en Al2O3 suffit pour d\216stabiliser entre 3 et 4 litres)Tj 0 -1.08333 TD [(de la solution de base. Ensuite, r\216par)-21(tir dans des b)-10(outeilles en)]TJ T* (verre marron pouvant \220tre ferm\216es et mettre en stock \210 +4 )Tj 25.95802 0.00001 TD Tj 0.38525 0.00001 TD (C)Tj -26.34327 -1.08336 TD [(\()-21(max. 5 jours)-21(\) ou entre \3201)104(5 \241)-31(C et \32020 \241)-31(C \()-42(jusqu\325\210 6 mois)-21(\).)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 198.8441 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62477 0.00001 TD 0 Tw (r\216-infiltration)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 190.9268 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Dans le r\216cipient en verre, m\216langer 200 ml de solution de base)Tj 0 -1.08333 TD -0.0005 Tc <88>Tj 0.8125 0 Td 0 Tc [(1)-169(g de durcisseur 1 \()-21(spatule en verre ou agitateur magn\216tique\),)]TJ -0.8125 -1.08334 TD [(jusqu\325\210 ce que le durcisseur soit enti\217rement dissout. P)21(r\216-infilt-)]TJ 0 -1.08333 TD (rer l\325\216chantillon pendant 1 h environ, \210 la temp\216rature ambiante.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 162.0921 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Infiltration)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 154.1748 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Dans le r\216cipient en verre, m\216langer 200 ml de solution de base)Tj 0 -1.08334 TD -0.0005 Tc <88>Tj 0.8125 0 Td 0 Tc [(1)-85-168(2)-169(g de durcisseur \()-21(agitateur magn\216tique\). Ajouter 20)-169(g de)]TJ -0.8125 -1.08334 TD (poudre et, \210 l\325aide de l\325agitateur magn\216tique, m\216langer jusqu\325\210)Tj 0 -1.08332 TD 0.0001 Tc (ce)Tj 1.33425 0 Td 0 Tc (que la poudre soit enti\217rement dissoute. Infiltrer l\325\216chantillon)Tj -1.33425 -1.08334 TD -0.0001 Tc <88>Tj 0.81283 0 Td (4)Tj 0.85392 0 Td 0 Tc [-31(C pendant 1 \210 24 heures.)-236(Dans le cas d\325\216chantillons de plus)]TJ -1.66675 -1.08334 TD [(grande taille \()-21(endoproth\217ses, par exemple\), il faut jusqu\325\210 48 h \210)]TJ 0 -1.08332 TD (des temp\216ratures plus basses.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 105.8402 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (R\216alisation de la solution m\217re A)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 97.9228 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(V)52(erser 400 ml de solution de base dans l\325\216prouvette \(500 ml)-21(\) et)]TJ 0 -1.08334 TD (ajouter progressivement 80 g de poudre. A l\325aide de l\325agitateur)Tj 0 -1.08332 TD (magn\216tique, remuer \210 la temp\216rature ambiante jusqu\325\210 ce que)Tj 0 -1.08334 TD (la poudre soit enti\217rement dissoute. La solution doit \220tre claire.)Tj T* (Ajouter le durcisseur 1)Tj 10.11002 -0.00002 TD [(\(3)-169(g ou 4)-169(g)-22(\) et terminer de remuer \210 l\325aide)]TJ -10.11002 -1.0833 TD (de l\325agitateur magn\216tique. Remplir ensuite de solution de base)Tj 0 -1.08334 TD (jusqu\325\210 500 ml.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 49.5882 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (R\216alisation de la solution m\217re B)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 41.6708 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Dans l\325\216prouvette de 50 ml, verser 4 ml de durcisseur 2 et )Tj 0 -1.08332 TD (remplir de 30 ml de solution de base. A l\325aide de l\325agitateur )Tj 0 -1.08334 TD [(magn\216tique, m\216langer un cour)-21(t instant. Ajouter ensuite 2 ml de )]TJ T* (r\216gulateur et remplir de solution de base jusqu\325\210 50 ml, puis)Tj 0 -1.08332 TD (rem\216langer avec l\325agitateur magn\216tique.)Tj -29.69293 17.13416 TD (D\216signation)Tj 14.64565 0.00002 TD (Quantit\216)Tj 8.50393 0.00002 TD (N)Tj 0.70825 0.00002 TD Tj 0.646 0.00002 TD (de)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 20.268 109.6371 m 189.496 109.6371 l S BT /F1 1 Tf 6 0 0 6 161.9997 111.9758 Tm (mat\216riau)Tj -23.14958 -1.55578 TD [(S)-16(olution de base )-6671(1)103(.)-1(0)-1(00)-169(g)-5419(1)]TJ 0 -1.08334 TD [(T)104(echnovit 9)83(1)103(00 stabilis\216e)]TJ 0 -1.31953 TD [(P)21(oudre PM)-42(MA T)104(echnovit 9)84(1)103(0)-1(0)-1333(1)104(2)-1(0)-169(g)-6189(2)]TJ 0 -1.31957 TD [(Durcisseur 1 T)104(echnovit 1)-3812(8 sachets de 1)-113(g)-1350(3)]TJ 0 -1.31955 TD [(Durcisseur 2 T)104(echnovit 9)83(1)104(0)-1(0)-2020(1)103(0 ml)-6128(4)]TJ 0 -1.31957 TD [(R\216gulateur de polym\216risation )-1542(5 ml)-6587(5)]TJ 0 -1.08332 TD (T)Tj 0.45848 0.00002 TD (echnovit 9)Tj 4.4585 0.00002 TD -0.104 Tc [(10)-105(0)]TJ ET 20.055 54.821 169.654 70.442 re s 107.7168 125.6422 m 107.7168 54.7752 l S 158.7403 125.6419 m 158.7403 54.7759 l S Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 255.0961 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Attention: doser le durcisseur 2 + et le r\216gulateur \210 l\325aide)Tj 0 -1.08333 TD (d\325une pipette!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 240.6787 Tm 0 Tr 0 0 0 1 k 0 Tc (V)Tj 0.55224 0.00001 TD 0 Tw (erser les solutions dans des b)Tj 13.09327 0.00001 TD (outeilles en verre marron, les )Tj -13.6455 -1.08335 TD (fermer et les identifier en cons\216quence. Entreposer entre )Tj 0 -1.08334 TD [(+4 \241)-31(C et \32020 \241)-31(C, en fonction des besoins!)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 218.3441 Tm 0 Tr 0 0 0 1 k 0 Tc (S)Tj 0.65575 0.00001 TD 0 Tw (olution de polym\216risation)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 210.4268 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(L)10(es solutions m\217res A et)-1( B)1( )-1(r)1(e)-1(froi)1(dies doivent \220tre m\216lang\216es )]TJ 0 -1.08333 TD (imm\216diatement avant l\325utilisation \210 l\325aide d\325une baguette en )Tj 0 -1.08334 TD [(verre, dans un rappor)-21(t de 9 par)-21(ts (v/v) de solution m\217re A)]TJ 0 -1.08333 TD (\()Tj 0.27099 0.00001 TD (b\216cher)Tj 3.06227 0.00001 TD -0.0001 Tc (\))Tj 0.54189 0 Td 0 Tc (et 1 par)Tj 3.41662 0.00001 TD (t)Tj 0.58405 0 Td [(de solution m\217re B \()-21(Pipette\) dans un b\216cher)42(.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 181.5921 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62475 -0.00001 TD 0 Tw (olym\216risation)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 173.6748 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (On recouvre ensuite lentement et jusqu\325au b)Tj 19.44703 0.00001 TD (ord les \216chantillons,)Tj -19.44703 -1.08335 TD (en position orient\216e, du m\216lange de polym\216risation, puis on cr\216e)Tj 0 -1.08333 TD [(un vide l\216ger)42(. R\216aliser le vide pendant 1)104(0 minutes environ, soit)]TJ T* (dans l\325exsiccateur pr\216-refroidi, \210 4 )Tj 15.10302 0.00002 TD Tj 0.38527 0.00002 TD 0.0001 Tc (C)Tj 1.00012 0 Td 0 Tc (\(vide l\216ger)Tj 4.41565 0.00001 TD 0.0001 Tc (,)Tj 0.50019 0 Td 0 Tc (p. ex. pompe)Tj -21.40425 -1.08339 TD [(de pulv\216risation d\325eau \210 200 m bar)-21(\), soit dans le cong\216lateur)42(, la)]TJ 0 -1.08334 TD [(pompe \210 vide \216tant raccord\216e \210 l\325ex)-10(t\216rieur)42(. La polym\216risation)]TJ 0 -1.08332 TD [(s\325effectue dans la plage de \3202 \210 \32020 \241)-31(C et doit \220tre termin\216e en)]TJ 0 -1.08334 TD [(24 heures. L)10(es temps de polym\216risation sont fonction du )]TJ T* [(volume de polym\216risation et de la temp\216rature. L)10(es \216chantillons)]TJ 0 -1.08332 TD (de plus grande taille doivent ainsi \220tre durcis \210 des temp\216ratures)Tj 0 -1.08334 TD [(plus basses. T)104(enir compte \210 cet \216gard de la capacit\216 frigorifique)]TJ T* [(du dispositif de refroidissement utilis\216 \()-21(compar)-21(timent \210 glace du)]TJ 0 -1.08332 TD (r\216frig\216rateur)Tj 5.26977 0.00002 TD 0.0001 Tc (,)Tj 0.50018 0 Td 0 Tc (cong\216lateur-coffre, cong\216lateur-armoire, caisson)Tj -5.76995 -1.08336 TD (de cong\216lation, p. ex. pour blocs de paraffine, avec fermeture )Tj 0 -1.08334 TD 0.0001 Tc <88>Tj 0.81315 0 Td 0 Tc (couvercle\). On obtient des r\216sultats reproductibles, pour )Tj -0.81315 -1.08332 TD (diff\216rentes tailles d\325\216chantillons, dans un cong\216lateur-armoire)Tj 0 -1.08334 TD (antid\216flagrant, \210 des temp\216ratures \210 r\216glage variable entre \3202 et )Tj T* [-31(C, \210 une constance de temp\216rature de +/\3200,5 \241)-31(C. Ne pas)]TJ 0 -1.08332 TD (ouvrir les r\216cipients pendant la polym\216risation.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 47.3402 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62475 0.00002 TD 0 Tw (oursuite de la mise en \317uvre)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 39.4228 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Une fois que les \216chantillons se sont r\216chauff\216s \210 la temp\216rature)Tj 0 -1.08334 TD (ambiante, apr\217s la polym\216risation, ils peuvent \220tre plac\216s sur)Tj 0 -1.08332 TD (des blocs avec des histoblocs et T)Tj 15.02075 0.00002 TD (echnovit 3040, afin de retirer)Tj -15.02075 -1.08336 TD (les \216chantillons du moule en t\216flon.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 666.1273 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(F)20(ur)-15(ther processing )]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 658.2099 Tm 0 Tr 0 0 0 1 k 0 Tc (O)Tj 0.75 -0.00001 TD 0 Tw (nce the specimens have reached room temperature after )Tj -0.75 -1.08331 TD 0.0002 Tc (p)Tj 0.58316 -0.00001 TD 0 Tc [(olymerisation, use Histoblocks or T)104(echnovit 3040 and remove)]TJ -0.58316 -1.08333 TD [(the specimens from the T)104(eflon moulds.)]TJ 0 -1.31955 TD [(After)-21(wards, the specimens are cut into sections, moistened with)]TJ 0 -1.08333 TD [(3)-1(0)-169(% ethanol throughout the procedure. All sections are placed)]TJ T* (on coated slides adding 9)Tj 11.35376 0.00001 TD (6)Tj 0.73125 0.00001 TD 0.0001 Tc (%)Tj 1.12511 0 Td 0 Tc (ethanol. Finally all sections are )Tj -13.21011 -1.08336 TD (covered with P)Tj 6.5415 0.00001 TD 0.0001 Tc (E)Tj 0.89619 0 Td 0 Tc (film, pressed overnight at 3)Tj 11.81158 0.00001 TD 0.0003 Tc (7)Tj 0.85425 0.00001 TD 0 Tc Tj 0.38525 0.00001 TD 0.0001 Tc (C)Tj 1.00021 0 Td 0 Tc (to 60 )Tj 2.56252 0.00001 TD Tj 0.38526 0.00001 TD 0.0001 Tc (C)Tj 1.00009 -0.00001 TD 0 Tc (and )Tj -25.43686 -1.08339 TD (deplastify prior to staining. )Tj 0 -1.31955 TD (Please refer to our brochure on our website )Tj 0 -1.08333 TD [(www)42(.kulzer-technik.com for fur)-20(ther information on fixation, )]TJ T* (dehydration, deplastification and routine staining procedures.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 581.0406 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(reparation Aids for T)104(echnovit 9)74(1)93(0)-1(0)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 573.1233 Tm 0 Tr 0 0 0 1 k -0.0001 Tc Tj 0.97976 0 Td 0 Tc 0 Tw [(T)104(eflon mould)]TJ -0.97976 -1.08333 TD 0.0001 Tc Tj 0.98021 0 Td 0 Tc (Histoblocks)Tj -0.98021 -1.08333 TD -0.0005 Tc Tj 0.979 0 Td 0 Tc [(P)20(olyethylene film)]TJ -0.979 -1.08333 TD 0.0001 Tc Tj 0.98016 0 Td 0 Tc (Specimen-levelling press)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 544.2888 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Danger/)-21(Safety advices)]TJ 0 -1.31957 TD (Please follow the instructions on the product packaging and )Tj 0 -1.08333 TD (safety data sheets)Tj 8.64599 0.00001 TD (.)Tj -8.64599 -1.08335 TD [(www)42(.kulzer-technik)-21(.com)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 515.454 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Our technical advice, whether verbal, in writing or by way of tri)Tj 27.12354 0.00001 TD (-)Tj -27.12354 -1.08335 TD [(als, is given in good faith but without warranty)42(, and this also ap-)]TJ 0 -1.08333 TD [(plies where proprietar)-21(y rights of third par)-21(ties are involved. It)]TJ T* (does not release you from the obligation to test the products)Tj 0 -1.08334 TD (supplied by us as and to their suitability for the intended proces-)Tj 0 -1.08333 TD [(ses and uses. T)10(he application, use and processing of the pro-)]TJ T* (ducts are beyond our control, and therefore, entirely your own)Tj 0 -1.08334 TD [(responsibility)42(. Should, in spite of this, liabilty be established for)]TJ 0 -1.08333 TD (any damage, it will be limited to the value of the goods delivered)Tj T* (by us and used by you. W)Tj 11.34375 0.00001 TD 0.0001 Tc (e)Tj 0.81322 0 Td 0 Tc (will, of course, provide consistent)Tj -12.15697 -1.08335 TD [(quality of our products within the scope of our G)-10(eneral)]TJ 0 -1.08333 TD [(Conditions of Sale and Deliver)-21(y)42(.)]TJ 0 -2.16667 TD [(*)-827(F)21(or more information please visit www)42(.kulzer-technik.com)]TJ 0 -1.83667 TD Tj 1.1811 -0.32999 TD (=)Tj 0.85404 0 Td [(registered trademark, K)10(ulzer GmbH)-21(, Hanau)]TJ ET /GS3 gs q 19.425 1057.323 34.016 22.6771 re W n /Fm0 Do Q q 19.842 237.346 34.016 22.677 re W n /Fm2 Do Q q 19.842 648.038 34.016 22.677 re W n /Fm4 Do Q Q endstream endobj 13 0 obj << /BitsPerSample 8 /Domain[0 1] /Size[255] /FunctionType 0 /Range[0 1 0 1 0 1 0 1] /Length 1020 >> stream                      !""#$$%&&' ( (!)!*"*#+#,$,$ -% .%!.&!/&"0'"0'# 1(# 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P)21(oi aggiungere 3 ml di regolatore e)]TJ -1.41619 -1.08331 TD (riempire fino a 50 ml con soluzione base, mescolare poi di )Tj 0 -1.08333 TD (nuovo con l\325agitatore magnetico.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 822.8571 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Attenzione: Dosare l\325indurente 2 e il regolatore con la )Tj 0 -1.08334 TD (pipetta!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 808.4398 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(V)52(ersare le soluzioni in b)-10(ottiglie di vetro scuro, chiudere e con-)]TJ 0 -1.08334 TD [(trassegnare. Stoccaggio a +4 \241)-31(C fino a \32020 \241)-31(C a seconda della)]TJ 0 -1.08332 TD (n)Tj 0.562 -0.00002 TD (ecessit\210!)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 786.1052 Tm 0 Tr 0 0 0 1 k 0 Tc (S)Tj 0.65577 0.00001 TD 0 Tw (oluzione per polimerizzazione )Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 778.1879 Tm 0 Tr 0 0 0 1 k 0 Tc (L)Tj 0.5525 0.00001 TD (e)Tj 0.813 0 Td 0 Tw [(soluzioni madre raffreddate A e B)-21(, immediatamente prima)]TJ -1.3655 -1.08334 TD [(dell\325uso in rappor)-21(to 9 par)-21(ti \(v/v\) di soluzione madre A \()-21(cilindro)]TJ T* [(graduato)-21(\) e 1 par)-21(te di soluzione madre B \()-21(pipetta)-21(\) devono )]TJ 0 -1.08333 TD (essere miscelate in un bicchiere di vetro con l\325aiuto di un )Tj T* (bastoncino di vetro.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 742.8532 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(olimerizzazione)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 734.9359 Tm 0 Tr 0 0 0 1 k 0.0002 Tc (I)Tj 0.52132 0 Td 0 Tc 0 Tw (campioni vengono poi riempiti fino all\325orlo in posizione orienta-)Tj -0.52132 -1.08334 TD [(ta con la miscela di polimeriz)-16(zazione e poi leggermente svuotati.)]TJ 0 -1.08332 TD [(Eseguire lo svuotamento per circa 1)104(0 minuti o in essiccatore)]TJ 0 -1.08334 TD [(preraffreddato a 4 \241)-31(C \()-21(leggero vuoto, p.es. con iniettore idraulico)]TJ 0.0001 Tc (a)' 0.81328 0 Td 0 Tc [(200 mbar)-21(\) oppure direttamente nel congelatore con pompa)]TJ -0.81328 -1.08332 TD [(del vuoto allacciata all\325esterno. La polimeriz)-16(zazione avviene nel)]TJ 0 -1.08334 TD [(range da \3202 a \32020 \241)-31(C e dovrebbe essere conclusa in 24 ore. I)]TJ T* [(tempi di polimeriz)-16(zazione dipendono dal volume di polimeriz)-16(za-)]TJ 0 -1.08332 TD (zione e dalla temperatura. I campioni di dimensioni maggiori )Tj 0 -1.08334 TD (devono essere quindi induriti a temperature pi\235 basse. In tal )Tj T* (caso occorre tenere conto della capacit\210 di raffreddamento del)Tj 0 -1.08332 TD [(dispositivo di raffreddamento utiliz)-16(zato \()-21(scompar)-21(to dei surgelati)]TJ 0 -1.08334 TD (nel congelatore, congelatore, freezer p.es. per blocchi di )Tj T* [(paraffina con coperchio a chiusura ermetica)-21(\). I risultati riproduci-)]TJ 0 -1.08332 TD [(bili per campioni di diverse grandez)-16(ze si raggiungono in un )]TJ 0 -1.08334 TD (congelatore antiesplosivo con temperature liberamente )Tj 0 -1.08333 TD [(impostabili tra \3202 e \32025 \241)-31(C con una costante termica di )]TJ 0 -1.08334 TD [(+/\3200,5 \241)-31(C. Non aprire i recipienti durante la polimeriz)-16(zazione.)]TJ -29.69295 23.87202 TD [(Denominazione)-7833(Quantit\210)-4560(Codice)]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 20.267 758.83 m 189.496 758.83 l S BT /F1 1 Tf 6 0 0 6 160.5824 761.168 Tm (materiale.)Tj -22.91339 -1.55576 TD [(T)104(echnovit 9)83(1)103(00 )-7853(1)104(.)-1(0)-1(0)-1(0)-169(g)-5183(1)]TJ 0 -1.08334 TD [(soluzione base stabiliz)-16(zata)]TJ 0 -1.31956 TD [(T)104(echnovit 9)83(1)103(00 polvere PM)-42(MA)-1332(1)103(20)-170(g)-5953(2)]TJ T* [(T)104(ech)-1(novit Induritore 1)-5166(8)-236(sacchetti)-236(da)-151(1)-45(g)-893(3)]TJ 0 -1.31954 TD (T)Tj 0.4585 0.00001 TD [(echnovit 9)83(1)104(00 Induritore 2)-2812(1)103(0 ml)-5892(4)]TJ -0.4585 -1.31956 TD [(T)104(echnovit 9)83(1)103(00 )-7853(5 ml)-6351(5)]TJ 0 -1.08333 TD [(regolatori polimeriz)-16(zazione)]TJ ET 20.055 704.014 169.654 70.441 re s 107.7163 774.6677 m 107.7163 703.8007 l S 157.3226 774.667 m 157.3226 703.801 l S BT /F1 1 Tf 6 0 0 6 286.7241 601.8698 Tm (Base)Tj 6.14175 0.00001 TD (P)Tj 0.62475 0.00001 TD (olvere)Tj 5.51697 0.00001 TD (Indurente 1)Tj 6.14173 0.00001 TD (Indurente 2)Tj 6.14173 0.00001 TD (Regolatore)Tj 6.14173 0.00001 TD (Stoccaggio/scadenza )Tj ET 201.685 593.031 m 552.329 593.031 l S BT /F1 1 Tf 6 0 0 6 470.9761 595.3697 Tm (delle soluzioni a:)Tj ET 201.685 583.697 m 552.329 583.697 l S BT /F1 1 Tf 6 0 0 6 204.5195 586.0352 Tm [(Codice componente)-4764(1)-5579(2)-5580(3)-5580(4)-5580(5)]TJ ET 201.685 574.3621 m 552.329 574.3621 l S BT /F1 1 Tf 6 0 0 6 204.5195 576.7004 Tm (P)Tj 0.62475 -0.00001 TD 0.0001 Tc (r)Tj 0.33315 -0.00001 TD 0 Tc [(einfiltrazione 3)-6431(200 ml)-9241(1)-169(g)-17132-31(C fino a sei mesi)]TJ ET 201.685 565.027 m 552.329 565.027 l S BT /F1 1 Tf 6 0 0 6 204.5195 567.3658 Tm (Infiltrazione)Tj 13.70077 0.00001 TD (ad 250 ml)Tj 6.14175 0.00001 TD 0.0004 Tc (2)Tj 0.56242 -0.00001 TD (0)Tj 0.85484 0 Td (g)Tj 4.72447 0.00002 TD 0 Tc [(1)-169(g)-169(/)-169(*)-1(*)-1(2)-169(g)-14438-31(C fino a sei mesi)]TJ ET 201.685 555.693 m 552.329 555.693 l S BT /F1 1 Tf 6 0 0 6 204.5195 558.0311 Tm (S)Tj 0.64112 0.00001 TD (oluzione madre A)Tj 13.05965 0.00001 TD (ad 500 ml)Tj 6.14175 0.00001 TD (80 g)Tj 6.14172 0.00001 TD (3)Tj 0.73125 0.00001 TD (g)Tj 0.73125 0.00001 TD (/)Tj 0.52299 0.00001 TD 0.001 Tc (**)Tj 0.7085 0.00001 TD 0 Tc (4)Tj 0.73125 0.00001 TD [(g)-14438-31(C fino a sei mesi)]TJ ET 201.685 546.358 m 552.329 546.358 l S BT /F1 1 Tf 6 0 0 6 204.5195 548.6965 Tm (S)Tj 0.64112 0.00001 TD [(oluzione madre B)-5310(ad 50 ml)-14550(4 ml)-4225(2 ml)-4224-31(C fino a sei mesi)]TJ -0.64112 -1.55579 TD (Miscela di )Tj 13.70077 0.00001 TD 0.0001 Tc (9)Tj 0.85425 0 Td 0 Tc (par)Tj 1.45825 0.00001 TD (ti di volume)Tj 18.94735 0.00001 TD (soluzione madre A)Tj -34.96062 -1.08337 TD [(polimeriz)-16(zazione)-18968(e)-17613(Utiliz)-16(zare immediatamente)]TJ 13.70077 -1.08333 TD 0.0001 Tc (1)Tj 0.85425 0 Td 0 Tc [(par)-21(te di volume)-13593(soluzione madre B)]TJ ET 201.472 524.042 351.07 84.6139 re s 283.4644 608.8688 m 283.4644 523.8298 l S 320.3153 608.8688 m 320.3153 546.5068 l S 357.1656 608.8693 m 357.1656 546.5072 l S 394.016 608.8687 m 394.016 546.5067 l S 430.8664 608.869 m 430.8664 546.507 l S 467.7164 608.8688 m 467.7164 523.8298 l S Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 515.0959 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Attenzione!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 507.1787 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(**S)-16(olo con l\325utiliz)-16(zo di T)104(echnovit 9)84(1)103(00 soluzione di base stabiliz)-16(zata vengono addizionati 2)-169(g \()-21(soluzione per infiltrazione\) e 4)-169(g )]TJ 0 -1.08333 TD (\()Tj 0.271 0.00001 TD (soluzione madre A)Tj 8.20825 0.00001 TD 0.0001 Tc (\))Tj 0.54222 0 Td 0 Tc (alla soluzione base.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 11 0 0 11 59.5276 469.5356 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Informaci\227n de uso)Tj 0 -1.09521 TD [(T)104(echnovit 9)73(1)94(00)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 442.6537 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (\347reas de aplicaci\227n)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 434.7364 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(e)-1(ch)1(no)-1(v)1(it 9)83(1)103(00 es un sistema de polimerizaci\227n sobre la base)]TJ 0 -1.08333 TD (de metilmetacrilato y ha sido desarrollado especialmente para el)Tj 0.0001 Tc (e)' 0.52108 -0.00001 TD 0 Tc (ncapsulado de tejidos minerales en la microscop\222a \227ptica.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 412.4017 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(ropiedades del material)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 404.4844 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(La polimerizaci\227n qu\222mica del hidr\227fob)-10(o T)105(echnovit 9)83(1)103(00 se )]TJ 0 -1.08333 TD (produce bajo exclusi\227n de ox\222geno con ayuda de un sistema)Tj T* [(catalizador de per\227xido y amina. L)10(os componentes adicionales)]TJ 0 -1.08334 TD [(como PM)-42(MA en polvo y reguladores posibilitan una polimeriza-)]TJ 0 -1.08333 TD [(ci\227n controlada en caso de fr\222o \()-21(en la gama de \3202 a \32020 \241)-31(C)-1(,)]TJ T* (seg\234n la cantidad)Tj 7.81225 0.00001 TD (\), que garantizan una disipaci\227n completa del)Tj -7.81225 -1.08335 TD (calor de polimerizaci\227n.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 356.1498 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Componentes T)Tj 7.31225 0.00001 TD (echnovit 9)Tj 4.78102 0.00001 TD (1)Tj 0.48925 0.00001 TD 0.001 Tc (00)Tj -12.58252 -14.00267 TD 0 Tc (\301Atenci\227n!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 264.2167 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(La soluci\227n b\207sica T)104(echnovit 9)83(1)104(00 puede ser empleada tanto)]TJ 0 -1.08333 TD [(estabilizada con tambi\216n desestabilizada. \301P)21(ara estudios inmun-)]TJ T* (ol\227gicos histoqu\222micos desestabilizar la soluci\227n b\207sica!)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 241.8821 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Desestabilizaci\227n de la soluci\227n b\207sica)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 233.9647 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Llenar la columna de cromatograf\222a con 50)-169(g Al2O3 \()-21(activo, )]TJ 0 -1.08333 TD [(b\207sico, 90\), hacer pasar lentamente la soluci\227n b\207sica T)104(echnovit)]TJ (9)' 0.4795 0.00001 TD (1)Tj 0.45848 0.00001 TD [(00 \()-21(n\234mero de componente 1)104(\). Una carga de columna con)]TJ -0.93798 -1.08336 TD [(Al2O3 alcanza para desestabilizar 3)-169-168(4 litros de soluci\227n b\207sica.)]TJ 0 -1.08333 TD 0.0001 Tc (A)Tj 0.93827 0 Td 0 Tc (continuaci\227n porcionar en b)Tj 12.38351 0.00001 TD (otellas con cierre de vidrio )Tj -13.32178 -1.08334 TD [(ambarino y mantener en reser)-21(va a +4 \241)-31(C \()-21(m\207x. 5 d\222as)-21(\) o a )]TJ T* [104(5 \241)-31(C hasta \32020 \241)-31(C \()-21(hasta )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 94.7195 194.9647 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 101.72 194.9647 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(a\226o)-21(\).)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 185.6301 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(reinfiltraci\227n)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 177.7128 Tm 0 Tr 0 0 0 1 k -0.006 Tc 0 Tw (En un recipiente de vidrio de 200 ml mezclar soluci\227n b\207sica con)Tj 0 -1.08333 TD -0.0055 Tc (1)Tj 0.84294 0 Td (g)Tj 0.84294 0 Td (de)Tj 1.3794 0 Td -0.006 Tc [(endurecedor 1 \()-21(esp\207tula de vidrio o agitador magn\216tico)-20(\))-1(,)]TJ -3.06527 -1.08333 TD (hasta que el endurecedor se haya disuelto completamente.)Tj 0 -1.08334 TD 0 Tc (P)Tj 0.61913 0.00001 TD -0.006 Tc (rinfiltrar la muestra aprox. 1 hora a temperatura ambiente.)Tj 59.85331 52.47581 TD 0 Tc (Resultados reproducibles para diferentes tama\226os de muestras)Tj 0 -1.08333 TD (se logran en un congelador ver)Tj 13.687 0.00001 TD (tical con protecci\227n contra )Tj -13.687 -1.08335 TD (explosiones con regulaci\227n variable de temperaturas entre \3202 )Tj 0 -1.08333 TD -0.0001 Tc (a)Tj 0.8129 0 Td 0 Tc (\32025 )Tj 2.10385 0.00001 TD Tj 0.38525 0.00001 TD (C)Tj 1.00008 0 Td (con una constante de temperatura de +/\3200,5 )Tj 20.12467 0.00001 TD Tj 0.38525 0.00001 TD (C. No)Tj -24.81201 -1.08337 TD (abrir los recipientes durante la polimerizaci\227n.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 437.7331 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(rocedimientos ulteriores)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 429.8158 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Una vez que las muestras tras la polimerizaci\227n se hayan )Tj 0 -1.08333 TD (templado a temperatura ambiente, se puede levantar en )Tj T* (bloques con histobloques y T)Tj 12.83325 0.00001 TD (echnovit 3040, para ex)Tj 9.98878 0.00001 TD (traer las)Tj -22.82203 -1.08336 TD [(muestras del molde de T)104(eflon.)]TJ 0 -1.31957 TD 0.0001 Tc (A)Tj 0.93811 0 Td 0 Tc [(continuaci\227n se realiza el cor)-21(te de las muestras, en donde )]TJ -0.93812 -1.08333 TD [(durante el cor)-21(te, el bloque se debe humectar con etanol al 30)-169(%)-1(.)]TJ (L)' 0.55249 0.00001 TD [(os cor)-21(tes ahora se retiran y se estiran sobre sopor)-21(tes de )]TJ -0.55249 -1.08335 TD [(objetos recubier)-21(tos con etanol al 96)-113(%. Finalmente los cor)-21(tes se)]TJ 0 -1.08333 TD (cubren con pel\222cula de P)Tj 10.99927 0.00001 TD (E)Tj 0.89607 0 Td (y)Tj 0.75008 0 Td (se prensan durante la noche a )Tj -12.64542 -1.08334 TD [(3)41(7 )1-32(C hasta 60 )1-32(C y desplastificados antes del coloreado.)]TJ 0 -1.31955 TD (Informaciones detalladas sobre fijaci\227n, deshidratado, desplasti-)Tj 0 -1.08333 TD (ficado y coloreado de rutina la encontrar\207 en nuestro cat\207logo o)Tj 0 -1.08334 TD [(bajo www)42(.kulzer-technik.com.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 339.6465 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Auxiliares de procesamiento para T)Tj 16.50023 0.00001 TD (echnovit 9)Tj 4.78102 0.00001 TD -0.094 Tc [(10)-95(0)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 331.7291 Tm 0 Tr 0 0 0 1 k 0.0001 Tc Tj 0.98012 0 Td 0 Tc 0 Tw (Molde de encapsulado)Tj -0.98012 -1.08333 TD 0 0 0 0 k Tj 0.97925 0.00001 TD 0 0 0 1 k (de T)Tj 1.85423 0.00001 TD (eflon)Tj -2.83349 -1.08336 TD 0.0002 Tc Tj 0.98031 0 Td 0 Tc (Histobloques)Tj -0.98031 -1.08333 TD -0.0005 Tc Tj 0.979 0 Td 0 Tc 0.001 Tw [(P)20(el\222culas)1( d)1(e p)1(ol)-1(i)1(et)-1(i)1(leno)]TJ -0.979 -1.08333 TD -0.0005 Tc Tj 0.979 0 Td (P)Tj 0.625 0.00001 TD 0 Tc 0 Tw (rensa por)Tj 4.20825 0.00001 TD (taobjetos)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 296.3945 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Indicaciones de peligros/de seguridad)Tj 0 -1.31955 TD [(P)21(or favor)42(, siga las instrucciones en el envase del producto y)]TJ 0 -1.08333 TD (en la ficha t\216cnica)Tj 8.58349 0.00001 TD -0.0002 Tc (.)Tj -8.58349 -1.08334 TD 0 Tc [(www)42(.kulzer-technik)-21(.com)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 267.5598 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Nuestra asesor\222a de aplicaciones t\216cnicas en base a palabras,)Tj 0 -1.08334 TD [(escritos y mediante ensayos se lleva a cab)-10(o conforme a nuestro)]TJ 0 -1.08333 TD (leal saber)Tj 4.14502 0.00001 TD 0.0001 Tc (,)Tj 0.50013 0 Td 0 Tc (sin embargo, s\227lo es valida a manera de recomenda-)Tj -4.64515 -1.08334 TD (ci\227n sin ning\234n compromiso, incluso con respecto a eventuales)Tj T* (derechos de protecci\227n de terceros, y no le exonera a usted de)Tj 0 -1.08333 TD (comprobar la idoneidad de los productos suministrados por no-)Tj T* (sotros para los procedimientos y fines pretendidos. Aplicaci\227n,)Tj 0 -1.08334 TD (uso y manipulaci\227n de los productos est\207n m\207s all\207 de nuestras)Tj 0 -1.08333 TD (posibilidades de control, siendo, por tanto, responsabilidad)Tj T* (exclusiva del usuario. Naturalmente, garantizamos la impecable)Tj 0 -1.08334 TD (calidad de nuestros productos de acuerdo a nuestras)Tj 0 -1.08333 TD [(Condiciones de V)52(enta y Suministro.)]TJ 0 -2.16667 TD (*)Tj 1.16667 0.00001 TD [(Informazioni in merito sono disponibili all'indiriz)-16(zo Internet)]TJ 0 -1.08334 TD [(www)42(.kulzer-technik.com)]TJ -1.16667 -1.83665 TD Tj 1.1811 -0.33002 TD 0.0001 Tc (=)Tj 0.85418 0 Td 0 Tc (marchio registrato, K)Tj 9.11481 -0.00001 TD [(ulzer GmbH)-21(, Hanau)]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 20.267 343.743 m 188.992 343.743 l S BT /F1 1 Tf 6 0 0 6 23.1021 346.0818 Tm [(Denominaci\227n)-8312(Cantidad)-3581(Material n\241)]TJ 0 -1.792 TD [(T)104(echnovit 9)83(1)103(00 )-7853(1)104(.)-1(0)-1(0)-1(0)-169(g)-4474(1)]TJ 0 -1.08333 TD (S)Tj 0.64112 0.00001 TD (oluci\227n b\207sica estabilizada)Tj -0.64112 -1.31956 TD (T)Tj 0.4585 0.00001 TD (echnovit 9)Tj 4.4585 0.00001 TD -0.104 Tc 7.958 Tw [(10)-104(0)-104( 12)-105(0)-273(g)-5349(2)]TJ -4.917 -1.08336 TD 0 Tc 0 Tw [(PM)-42(MA en polvo)]TJ 0 -1.31954 TD (T)Tj 0.4585 0.00001 TD (echnovit Endurecedor 1)Tj 14.18717 0.00001 TD 0.0003 Tc (8)Tj 0.8545 0 Td (b)Tj 0.59302 0.00001 TD 0 Tc (olsas de )Tj 3.9375 0.00001 TD (1)Tj 0.675 0.00001 TD (g)Tj 1.49903 0.00001 TD (3)Tj -22.20472 -1.31961 TD (T)Tj 0.4585 0.00001 TD (echnovit 9)Tj 4.4585 0.00001 TD (1)Tj 0.4585 0.00001 TD (00 Endurecedor 2)Tj 9.27017 0.00001 TD (1)Tj 0.4585 0.00001 TD 0.0003 Tc (0)Tj 0.8545 0 Td 0 Tc (ml)Tj 6.24605 0.00001 TD (4)Tj -22.20472 -1.31963 TD [(T)104(echnovit 9)83(1)103(00 )-7853(5 ml)-5642(5)]TJ 0 -1.08334 TD (Regulador de polimerizaci\227n)Tj ET 20.055 281.01 169.15 71.859 re s 107.7168 353.0824 m 107.7168 280.7994 l S 153.071 353.0827 m 153.071 280.7987 l S BT /F1 1 Tf 0 6 -6 0 12.2432 14.1729 Tm 0.0006 Tc (6)Tj 0.5626 0 TD (6)Tj 0.5626 0 TD (0)Tj 0.5626 0 TD (6)Tj 0.5626 0 TD (2)Tj 0.52 0 TD 0 Tc (7)Tj 0.5315 0 TD 0.0005 Tc (5)Tj 0.5625 0 TD (0)Tj 0.5625 0 TD (_)Tj 0.5005 0 TD (1)Tj 0.458 0 TD 0 Tc (1)Tj 0.88327 0 TD (D)Tj 0.68804 0 TD (.)Tj 0.20804 0 TD (O)Tj 0.75004 0 TD (.)Tj 0.20804 0 TD (G)Tj 0.781 0 TD 0.0002 Tc (.)Tj 0.5296 0 TD (5)Tj 0.56221 0 TD (-)Tj 0.37521 0 TD (s)Tj 0.47921 0 TD (p)Tj 0.58321 0 TD (r)Tj 0.291 0 TD 0.0001 Tc (.)Tj 0.52943 0 TD (/)Tj 0.67543 0 TD (0)Tj 0.47897 0 TD 0 Tc (1)Tj 0.4585 0 TD (/)Tj 0.25025 0 TD (1)Tj 0.4585 0 TD (7)Tj 6 0 0 6 289.5588 140.4014 Tm [(Base)-3934(P)22(olvo)-1836(Endurecedor 1)-1017(Endurecedor 2)-1018(Regulador)-1600(Almacenaje/)-42(Estabilidad )]TJ ET 201.685 131.563 m 552.329 131.563 l S BT /F1 1 Tf 6 0 0 6 479.48 133.9014 Tm (de las soluciones a:)Tj ET 201.685 122.228 m 552.329 122.228 l S BT /F1 1 Tf 6 0 0 6 204.5195 124.5668 Tm [(N\234mero de componente)-3403(1)-5579(2)-3690(3)-6997(4)-6997(5)]TJ ET 201.685 112.894 m 552.329 112.894 l S BT /F1 1 Tf 6 0 0 6 204.5195 115.2321 Tm [(P)21(reinfiltraci\227n 3)-7382(200 ml)-7352(1)-169(g)-19966-32(C hasta )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 516.4175 115.2321 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 523.418 115.2321 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (a\226o)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 103.5591 m 552.329 103.5591 l S BT /F1 1 Tf 6 0 0 6 204.5195 105.8975 Tm [(Infiltraci\227n)-9694(ad 250 ml)-1704(20 g)-2273(1)-169(g)-169(/)-169(*)-1(*)-1(2)-169(g)-17272-32(C hasta )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 516.4175 105.8975 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 523.418 105.8975 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (a\226o)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 94.224 m 552.329 94.224 l S BT /F1 1 Tf 6 0 0 6 204.5195 96.563 Tm [(S)-16(oluci\227n original A)-5949(ad 500 ml)-1704(80 g)-2273(3)-169(g)-169(/)-169(*)-1(*)-1(4)-169(g)-17272-32(C hasta )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 516.4175 96.563 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 523.418 96.563 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (a\226o)Tj ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 84.89 m 552.329 84.89 l S BT /F1 1 Tf 6 0 0 6 204.5195 87.2283 Tm [(S)-16(oluci\227n original B)-5907(ad 50 ml)-14078(4 ml)-5642(2 ml)-4225-31(C hasta )]TJ ET Q /GS3 gs /GS2 gs q BT /F2 1 Tf 6 0 0 6 516.4175 87.2283 Tm 0 Tr 0 0 0 1 k 0 Tc (|)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 523.418 87.2283 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (a\226o)Tj -53.14976 -1.55576 TD [(Mezcla de polimerizaci\227n )-2465(9 par)-21(tes por volumen)-11392(S)-16(oluci\227n original A)]TJ 0 -1.08334 TD [(polimeriz)-16(zazione)-20566(y)-17495(Emplear inmediatamente)]TJ 14.17322 -1.08334 TD 0.0001 Tc (1)Tj 0.85425 0 Td 0 Tc [(par)-21(te di volume)-13121(S)-16(oluci\227n original B)]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.472 62.5739 351.07 84.6141 re s 286.2997 147.4009 m 286.2997 62.3619 l S 323.1494 147.4009 m 323.1494 85.039 l S 348.6612 147.4017 m 348.6612 85.0387 l S 394.0156 147.401 m 394.0156 85.039 l S 439.3699 147.4013 m 439.3699 85.0393 l S 476.2209 147.4011 m 476.2209 62.3621 l S Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 54.3364 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Attenzione!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 46.4191 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(**S)-16(olo con l\325utiliz)-16(zo di T)104(echnovit 9)84(1)103(00 soluzione di base stabiliz)-16(zata vengono addizionati 2)-169(g \()-21(soluzione per infiltrazione\) e 4)-169(g )]TJ 0 -1.08332 TD [(\()-21(soluzione madre A)-21(\) alla soluzione base.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 473.034 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Infiltraci\227n)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 465.1167 Tm 0 Tr 0 0 0 1 k 0.0001 Tc (E)Tj 0.6041 -0.00001 TD (n)Tj 0.8542 0 Td 0 Tc 0 Tw (un recipiente de vidrio mezclar 200 ml de soluci\227n b\207sica)Tj -1.45831 -1.08332 TD (con 1 a 2 g de endurecedor)Tj 12.27003 0.00001 TD (.)Tj 0.5 0 Td (\()Tj 0.27099 0.00001 TD [(Agitador magn\216tico)-21(\) Adicionar)]TJ -13.04101 -1.08336 TD [(20)-170(g de polvo y mezclar con el agitador magn\216tico hasta que el)]TJ 0 -1.08333 TD 0.0001 Tc (p)Tj 0.58308 -0.00001 TD 0 Tc [(olvo se haya disuelto por completo. Infiltrar la muestra a 4 \241)-31(C )]TJ -0.58308 -1.08332 TD 0.0001 Tc (1)Tj 0.85419 0 Td (h)Tj 0.85419 0 Td 0 Tc [(hasta 24 h. En muestras mayores p.)-169(ej. endopr\227tesis son )]TJ -1.70839 -1.08334 TD (necesarias hasta 48 h a bajas temperaturas.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 423.2821 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Elab)-10(oraci\227n de soluci\227n b\207sica A)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 415.3647 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(reparar 400 ml de soluci\227n b\207sica en el matraz aforado \(500)]TJ 0 -1.08333 TD 0.001 Tc (ml)Tj 1.0835 0.00001 TD 0.0001 Tc (\))Tj 0.54214 0 Td (y)Tj 0.75014 0 Td 0 Tc (adicionar paso a paso 8)Tj 10.56149 0.00001 TD (0)Tj 0.73124 0.00001 TD 0.0001 Tc (g)Tj 0.85424 0 Td 0 Tc (de polvo. Con el agitador )Tj -14.52274 -1.08337 TD (magn\216tico a temperatura ambiente, agitar hasta la disoluci\227n)Tj 0 -1.08333 TD (completa del polvo. La soluci\227n debe ser transparente.)Tj T* (Adicionar endurecedor 1 \()Tj 11.43653 0.00001 TD [(3)-169(g o bien 4)-169(g)-22(\) y ultimar la agitaci\227n)]TJ -11.43653 -1.08335 TD (con el agitador magn\216tico. A continuaci\227n completar con solu-)Tj 0 -1.08333 TD (ci\227n b\207sica hasta 500 ml.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 367.0302 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Elab)Tj 2.05177 0.00001 TD (oraci\227n de soluci\227n b\207sica B)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 359.1129 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62477 0.00001 TD 0 Tw (reparar en el matraz aforado de 50 ml, 4 ml de endurecedor 2)Tj -0.62477 -1.08335 TD 0.0001 Tc (y)Tj 0.75018 0 Td 0 Tc (completarlo con 30 ml de soluci\227n b\207sica. Mezclar brevemen-)Tj -0.75018 -1.08333 TD (te con el agitador magn\216tico. A continuaci\227n adicional 2 ml de)Tj T* (regulador y completar con soluci\227n b\207sica hasta 50 ml y mez)Tj 26.97852 0.00001 TD (-)Tj -26.97852 -1.08335 TD (clar una vez m\207s con el agitador magn\216tico.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 323.7781 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Atenci\227n: \301Dosificar endurecedor 2 + regulador)Tj 21.93702 0.00001 TD 0.0002 Tc (,)Tj 0.54233 0 Td 0 Tc (con pipeta!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 315.8608 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Envasar las soluciones en b)Tj 12.19726 0.00001 TD (otellas de vidrio ambarino, cerrar e)Tj -12.19726 -1.08334 TD [(identificar adecuadamente. \301Almacenaje a +4 \241)-31(C hasta \32020 \241)-32(C)]TJ T* (seg\234n necesidad!)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 293.5262 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(S)-10(oluci\227n de polimerizaci\227n )]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 285.6088 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Las soluciones originales A y B enfriadas deben ser mezcladas)Tj 0 -1.08333 TD (inmediatamente antes de su uso, en relaci\227n de 9 par)Tj 23.62452 0.00001 TD (tes (v/v))Tj -23.62452 -1.08335 TD [(de soluci\227n original A \()-21(probeta graduada)-21(\) y una par)-21(te de )]TJ 0 -1.08333 TD (soluci\227n original B \()Tj 8.66652 0.00001 TD (pipeta)Tj 2.729 0.00001 TD 0.0001 Tc (\))Tj 0.54215 0 Td 0 Tc (en una copa de vidrio, con ayuda )Tj -11.93766 -1.08335 TD (de una varilla de vidrio.)Tj ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 250.2742 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(olimerizaci\227n)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 242.3569 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Las muestras a continuaci\227n se cubren lentamente con posi-)Tj 0 -1.08333 TD [(ci\227n orientada con la mezcla de polimerizaci\227n hasta el b)-10(orde y)]TJ (a)' 0.8131 0 Td (continuaci\227n se evac\234an ligeramente. Ejecutar la evacuaci\227n,)Tj -0.8131 -1.08333 TD (ya sea con secador preenfriado a 4 )Tj 15.79078 0.00001 TD Tj 0.38525 0.00001 TD (C)Tj 1 0 Td (\()Tj 0.271 0.00001 TD (ligero vac\222o, p.ej. inyec)Tj 9.74877 0.00001 TD (-)Tj -27.1958 -1.08338 TD [(tor de agua a 200 mbar)-21(\) o en el congelador con b)-10(omba de)]TJ 0 -1.08333 TD [(vac\222o ex)-10(terna conectada, durante aprox. 1)104(0 minutos. La polime-)]TJ T* (rizaci\227n se realiza en la gama de \3202 a \32020 )Tj 19.06202 0.00001 TD Tj 0.38525 0.00001 TD (C)Tj 1 0 Td (y)Tj 0.75 0 Td (debe estar con)Tj 6.58302 0.00001 TD (-)Tj -27.78028 -1.08337 TD [(cluida en 24 horas. L)10(os tiempos de polimerizaci\227n dependen)]TJ 0 -1.08333 TD (del volumen de esta as\222 como de la temperatura. De este modo,)Tj T* (muestras mayores deben ser curadas a temperaturas m\207s ba)Tj 27.02027 0.00001 TD (-)Tj -27.02027 -1.08335 TD (jas. En este caso se debe obser)Tj 13.9375 0.00001 TD (var la capacidad de fr\222o de la in-)Tj -13.9375 -1.08334 TD (stalaci\227n de refrigeraci\227n empleada \()Tj 16.18628 0.00001 TD [(compar)-21(timiento congelador)]TJ -16.18628 -1.08334 TD [(en la nevera, congelador horizontal, congelado ver)-21(tical, bandeja )]TJ T* (congeladora p.ej. para bloques de parafina con tapa de cierre\).)Tj -30.23622 152.87025 TD [(V)10(ient ensuite la coupe des \216chantillons, le bloc devant \220tre )]TJ 0 -1.08334 TD [(humidifi\216 avec de l\325\216thanol \210 30)-169(% pendant la coupe. On pr\216l\217ve)]TJ 0 -1.08332 TD [(alors les coupes et on les \216tale sur des por)-21(te-objets rev\220tus,)]TJ 0 -1.08334 TD [(avec de l\325\216thanol \210 96)-169(%. On comprime enfin les coupes, en les)]TJ T* [(recouvrant d\325un film en P)-21(E pendant la nuit, \210 3)42(7)-180-31(C \32060 \241)-31(C, et on)]TJ 0 -1.08332 TD (les d\216plastifie avant la coloration. Des informations d\216taill\216es sur)Tj 0 -1.08334 TD (la fixation, la d\216shydratation, la d\216plastification et les colorations)Tj T* (de routine sont donn\216es dans notre prospectus ou \210 l\325adresse)Tj 0 -1.08332 TD [(www)42(.kulzer-technik.com.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 1013.7439 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Aides \210 la mise en \317uvre pour T)104(echnovit 9)73(1)94(0)-1(0)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 1005.8265 Tm 0 Tr 0 0 0 1 k 0.0001 Tc Tj 0.98027 0 Td 0 Tc 0 Tw (Moule d\325inclusion en t\216flon)Tj -0.98027 -1.08334 TD 0.0001 Tc Tj 0.98023 0 Td 0 Tc (Histoblocs)Tj -0.98023 -1.08334 TD 0.0001 Tc Tj 0.98012 0 Td 0 Tc (Films de poly\216thyl\217ne)Tj -0.98012 -1.08332 TD -0.0005 Tc Tj 0.979 0 Td 0 Tc [(P)21(resse por)-21(te-objets)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 19.8425 976.9918 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Conseils de s\216curit\216/)-42(Indications de danger)]TJ 0 -1.31955 TD [(V)63(euillez vous r\216f\216rer \210 l'emballage du produit et/ou sa fiche)]TJ 0 -1.08333 TD (de donn\216es de s\216curit\216. )Tj 0 -1.08334 TD [(www)42(.kulzer-technik)-21(.com)]TJ ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 19.8425 948.1572 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Notre consultation sur la mani\217re d\325application, sous forme ora-)Tj 0 -1.08333 TD (le, \216crite et par des essais est effectu\216e au mieux de nos con-)Tj T* (naissances, mais n\325est valable cependant que comme indication)Tj 0 -1.08334 TD [(n\325entra\224nant aucune obligation, de m\220me par rappor)-21(t \210 des dro-)]TJ 0 -1.08333 TD (its de protection \216ventuels de tierces personnes, et ne vous lib\217-)Tj 43.06295 16.77835 TD [(Basis)-3767(P)22(o)-1(ud)1(re)-2955(Durcisseur)-235(1)-657(Durcisseur)-236(2)-657(R\216gulateur)-1371(Stockage/conser)-21(vation des )]TJ ET 0 J 0 j 10 M []0 d 0.425 w 0 0 0 1 K 201.685 1013.989 m 552.331 1013.989 l S BT /F1 1 Tf 6 0 0 6 425.6218 1016.3273 Tm (solutions \210:)Tj ET 201.685 1004.654 m 552.331 1004.654 l S BT /F1 1 Tf 6 0 0 6 204.5195 1006.9926 Tm [(Num\216ro de composant)-2117(1)-5580(2)-5579(3)-5580(4)-5580(5)]TJ ET 201.685 995.32 m 552.331 995.32 l S BT /F1 1 Tf 6 0 0 6 204.5195 997.658 Tm [(P)21(r\216infiltration 3)-5721(200 ml)-9242(1)-169(g)-17132-31(C: jusqu\325\210 6 mois)]TJ ET 201.685 985.985 m 552.331 985.985 l S BT /F1 1 Tf 6 0 0 6 204.5195 988.3234 Tm [(Infiltration)-8034(ad 250 ml)-1704(20 g)-4162(1)-169(g)-169(/)-169(*)-1(*)-1(2)-169(g)-14438-31(C: jusqu\325\210 6 mois)]TJ ET 201.685 976.65 m 552.331 976.65 l S BT /F1 1 Tf 6 0 0 6 204.5195 978.9888 Tm [(S)-16(olution m\217re A)-5226(ad 500 ml)-1704(80 g)-4162(3)-169(g)-169(/)-169(*)-1(*)-1(4)-169(g)-14438-31(C: jusqu\325\210 6 mois)]TJ ET 201.685 967.316 m 552.331 967.316 l S BT /F1 1 Tf 6 0 0 6 204.5195 969.6541 Tm [(S)-16(olution m\217re B)-5184(ad 50 ml)-14550(4 ml)-4225(2 ml)-4225-31(C: jusqu\325\210 6 mois)]TJ 0 -1.55579 TD [(M\216lange de )-6846(9 par)-21(ts en volume)-10508(S)-16(olution m\217re A)]TJ 0 -1.08333 TD [(polym\216risation)-18505(et)-17321(Utiliser imm\216diatement)]TJ 12.28345 -1.08333 TD 0.0001 Tc (1)Tj 0.85425 -0.00002 TD 0 Tc [(par)-21(t en volume)-10987(S)-17(olution m\217re B)]TJ ET 201.472 945 351.071 84.6139 re s 274.9608 1029.8265 m 274.9608 944.7875 l S 311.8106 1029.8264 m 311.8106 967.4644 l S 348.661 1029.8268 m 348.661 967.4638 l S 385.5114 1029.8262 m 385.5114 967.4642 l S 422.3618 1029.8265 m 422.3618 967.4646 l S 459.2128 1029.8264 m 459.2128 944.7875 l S Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 201.2598 936.0536 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Attention!)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 201.2598 928.1362 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(**N\325ajouter 2)-169(g \()-21(solution d\325infiltration)-21(\) et 4 \()-21(solution m\217re A)-21(\) \210 la solution de base que lorsque l\325on utilise la solution de base T)104(echnovit)]TJ 0 -1.08333 TD [(9)83(1)103(00 stabilis\216e.)]TJ ET Q /GS3 gs /GS2 gs q BT /F0 1 Tf 6 0 0 6 382.6772 894.9101 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Ulteriore trattamento)Tj ET Q /GS3 gs /GS2 gs q BT /F1 1 Tf 6 0 0 6 382.6772 886.9927 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Una volta che i campioni dopo la polimeriz)-16(zazione, si sono )]TJ 0 -1.08333 TD (riscaldati a temperatura ambiente, si pu\230 procedere con)Tj T* [(blocchetti di suppor)-21(to Histobloc e T)104(echnovit 3040 per prelevare)]TJ 0 -1.08334 TD 0.0001 Tc (i)Tj 0.50016 0 Td 0 Tc (campioni dalla forma di teflon.)Tj -0.50017 -1.31955 TD (Successivamente avviene il taglio dei campioni e durante il taglio)Tj 0 -1.08332 TD [(occorre inumidire il blocco con etanolo al 30)-169(%. I tagli vengono)]TJ 0 -1.08334 TD 0.0001 Tc (o)Tj 0.56208 -0.00002 TD 0 Tc [(ra eseguiti e stesi su por)-21(taoggetti rivestito con etanolo al 90)-169(%)-1(.)]TJ -0.56208 -1.08332 TD [(Infine i tagli vengono coper)-21(ti con pellicola in P)-21(E)-21(, pressati per )]TJ T* [(tutta la notte ad una temperatura da 3)42(7 \241)-31(C fino a 60 \241)-31(C e )]TJ 0 -1.08334 TD (disidratati prima della colorazione. 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