%PDF-1.3 % 1 0 obj << /OPM 1 /Type/ExtGState >> endobj 2 0 obj << /FL 1 /Type/ExtGState /SM 0.01 >> endobj 3 0 obj << /Type/ExtGState /SA true >> endobj 4 0 obj << /Name/Fm1 /BBox[-1.17593 -22.09825 15.62302 5.27746] /Subtype/Form /Resources<>>> /Type/XObject /Length 1266 /Matrix[1 0 0 1 0 0] /OPI<> >> /FormType 1 >> stream /GS1 gs q 0 0 1 1 re W n 0 0 0 0 k 0.983 0.5 m 0.983 0.2392 0.7667 0.0278 0.5 0.0278 c 0.2333 0.0278 0.017 0.2392 0.017 0.5 c 0.017 0.7608 0.2333 0.9722 0.5 0.9722 c 0.7667 0.9722 0.983 0.7608 0.983 0.5 c f /GS2 gs 0 J 0 j 4 M []0 d 0.0435 w 0 0 0 1 K 0.983 0.5 m 0.983 0.2392 0.7667 0.0278 0.5 0.0278 c 0.2333 0.0278 0.017 0.2392 0.017 0.5 c 0.017 0.7608 0.2333 0.9722 0.5 0.9722 c 0.7667 0.9722 0.983 0.7608 0.983 0.5 c s 0 0 0 1 k 0.2764 0.3201 m 0.3327 0.3201 l 0.3623 0.3208 0.3861 0.3255 0.4052 0.3691 c 0.4157 0.3932 0.4258 0.4297 0.4258 0.4966 c 0.4258 0.513 0.4262 0.5884 0.3957 0.6343 c 0.3885 0.6444 0.379 0.6538 0.3704 0.6592 c 0.3585 0.667 0.347 0.6685 0.3217 0.6685 c 0.2764 0.6685 l 0.2764 0.3201 l h 0.3303 0.7689 m 0.3413 0.7689 0.3518 0.7681 0.3628 0.7673 c 0.3928 0.7634 0.4172 0.7525 0.4415 0.7222 c 0.4978 0.6514 0.5002 0.534 0.5002 0.5013 c 0.5002 0.4756 0.4983 0.4499 0.4974 0.4422 c 0.4931 0.3854 0.4835 0.338 0.4596 0.2944 c 0.4525 0.2812 0.4367 0.2524 0.4095 0.236 c 0.3823 0.2189 0.3546 0.2205 0.3265 0.2197 c 0.2033 0.2197 l 0.2033 0.7689 l f 0.805 0.6709 m 0.6241 0.6709 l 0.6241 0.5503 l 0.7903 0.5503 l 0.7903 0.4562 l 0.6241 0.4562 l 0.6241 0.3208 l 0.8084 0.3208 l 0.8084 0.2197 l 0.5511 0.2197 l 0.5511 0.7689 l 0.805 0.7689 l f Q endstream endobj 5 0 obj << /Name/Fm0 /BBox[0 0 566.92896 566.92896] /Subtype/Form /Resources<>/ProcSet[/PDF]/ExtGState<>>> /Type/XObject /Length 58 /Matrix[1 0 0 1 0 0] /OPI<> >> /FormType 1 >> stream /GS1 gs q 33.7479 0 0 20.7092 39.685 457.637 cm /Fm1 Do Q endstream endobj 6 0 obj << /OP true /Type/ExtGState >> endobj 7 0 obj << /OP false /Type/ExtGState >> endobj 8 0 obj << /Length 18852 >> stream /GS0 gs q 0 0.929 566 566 re W n 1 g 0 566.929 0 0 re f Q /GS1 gs q 36.85 456.378 39.085 24.803 re W n /Fm0 Do Q /GS3 gs q BT /F0 1 Tf 11.75 0 0 11.75 78.6614 463.3376 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Gebrauchsinformation T)104(echnovit 8)73(1)94(0)-1(0)]TJ ET 0 0 0 0.1 k -8.504 -8.504 36.851 583.937 re f 0 0 0 0.7 k -8.504 491.811 583.937 4.252 re f Q /GS4 gs /GS3 gs q BT /F0 1 Tf 28 0 0 28 233.8583 514.9007 Tm 0 Tr 0 0 0 1 k -0.073 Tc 0 Tw [(81)20(0)-74(0)]TJ ET 0 0 0 0 k 39.685 11.926 161.575 427.444 re f Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 433.0159 Tm 0 Tr 0 0 0 1 k 0 Tc (S)Tj 0.63501 -0.00001 TD 0 Tw (chnitte speziell f\237r die Immunhistochemie)Tj -0.63501 -2.23076 TD [(T)103(echnovit 8)84(1)103(00 ist ein K)10(unststoffeinbettsystem auf Basis)]TJ 0 -1.11538 TD [(von H)-42(E)-42(MA f\237r lichtmikroskopische Untersuchungen. )]TJ T* [(Es eignet sich zum Einbetten von allen G)-10(eweben in)]TJ 0 -1.11539 TD [(Medizin, Zoologie und B)-10(otanik. S)-10(chnitte von entkalk)-16(ten)]TJ 0 -1.11538 TD [(oder kurzentkalk)-16(ten Beckenkammbiopsien und implan-)]TJ T* (tier)Tj 1.375 0.00001 TD (te Biomaterialien k\232nnen nicht nur f\237r histologische)Tj -1.375 -1.11539 TD [(F)21(\212rbungen genutz)-16(t werden, sondern auch f\237r Enz)-16(ym-)]TJ 0 -1.11538 TD (und Immunhistochemie.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 357.6813 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Materialeigenschaften)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 349.014 Tm 0 Tr 0 0 0 1 k 0 Tc (T)Tj 0.45851 0 TD 0 Tw [(echnovit 8)83(1)104(00 ist eine K)16(ombination von einem nahezu)]TJ -0.45851 -1.11539 TD [(geruchsfreien W)31(eichmacher und einem hydrophilen)]TJ 0 -1.11538 TD [(K)10(unststoff. T)104(echnovit 8)84(1)103(00 ist speziell f\237r die K\212ltepoly-)]TJ T* (merisation \(+)Tj 5.64575 0 TD [(4)-169-32(C\) entwickelt worden.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 317.1793 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(W)31(\212hrend der H\212r)-16(tung muss die Einbettform absolut)]TJ 0 -1.11539 TD [(luftdicht verschlossen sein)-21(, da das P)21(olymerisations-)]TJ T* [(system sauerstoffempfindlich ist)-21(.)]TJ 0 -1.98757 TD [(Die V)63(or)-16(teile auf einen Blick)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 282.0927 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 281.0927 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Reproduzierbarkeit und Zuverl\212ssigkeit der)Tj 0 -1.11539 TD (Einbettungen durch st\212ndige und dokumentier)Tj 20.5205 0 TD -0.001 Tc (te)Tj -20.5205 -1.11539 TD 0 Tc [(Qualit\212tskontrollen der einzelnen K)16(omponenten)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 258.9254 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 257.9254 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Niedrige P)21(olymerisationstemperatur von)-235(1)103(0)-169-31(C bis 0)-169-32(C)]TJ 0 -1.11538 TD (durch das spezielle Katalysatorsystem und die)Tj T* [(T)103(eflonformen)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 235.7581 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 234.7581 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Uniforme H\212r)-21(te des Blockes, dadurch gleichm\212\247ige,)]TJ 0 -1.11538 TD (d\237nnste S)Tj 4.48926 0 TD (chnitte)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 219.8407 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 218.8407 Tm 0 Tr 0 0 0 1 k 0 Tc (G)Tj 0.76002 0 TD 0 Tw (eringe S)Tj 3.63477 0.00001 TD (chrumpfungsar)Tj 6.729 0 TD (tefak)Tj 2.12012 0 TD [(te, deshalb her)-21(vorragen-)]TJ -13.24391 -1.1154 TD [(de Morphologie der G)-10(ewebe)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 203.9234 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 202.9234 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Routinef\212rbungen, Enz)-16(ymnachweise und)]TJ 0 -1.11538 TD (Immunhistochemie m\232glich)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 188.0061 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 187.0061 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (H\212matologische Beckenkammbiopsien m\237ssen nicht)Tj 0 -1.11538 TD [(entkalk)-16(t werden)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 172.0888 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 171.0888 Tm 0 Tr 0 0 0 1 k 0 Tc (G)Tj 0.76002 0 TD 0 Tw (eringe T)Tj 3.45825 0.00001 TD (oxizit\212t durch spezielle K)Tj 10.85865 0 TD (ombination von)Tj -15.07691 -1.1154 TD (W)Tj 0.86475 0 TD (eichmacher und Katalysatorsystem)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 153.7541 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Anwendung)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 145.0868 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)103(echnovit 8)84(1)103(00 der Step-by-)-31(Step-Anleitung entspre-)]TJ 0 -1.11538 TD (chend ansetzen. Die fixier)Tj 11.22877 0.00001 TD [(ten und dehydrier)-21(ten P)21(roben)]TJ -11.22877 -1.11539 TD [(in die Infiltrationsl\232sung legen. Eine niedrige T)104(emperatur)]TJ T* [(und Agitation der P)21(roben w\212hrend des gesamten)]TJ 0 -1.11538 TD (Einbettvorgangs sind vor)Tj 10.89575 0 TD (teilhaft.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 106.0022 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Die folgende Anleitung zur Fixierung und Dehydrie-)Tj 0 -1.11538 TD [(rung ist nicht z)-16(wingend er)-16(forderlich)-21(. T)104(echnovit 8)73(1)94(0)-1(0)]TJ T* (kann auch nach einer anderen V)Tj 15.06177 0.00001 TD (orbehandlung infil)Tj 8.43726 0.00001 TD (-)Tj -23.49903 -1.1154 TD (trier)Tj 1.93286 0 TD 0.0001 Tc (t)Tj 0.64615 0 Td 0 Tc (und polymerisier)Tj 7.82822 0 TD 0.0002 Tc (t)Tj 0.64635 0 Td 0 Tc [(werden)-21(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 74.1675 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(F)21(\237r den ganzen P)21(rozess sind dichtschlie\247ende G)-10(las-)]TJ 0 -1.11538 TD (oder P)Tj 2.95826 0.00001 TD (E)Tj 0.61426 0.00001 TD (-)Tj 0.385 0.00001 TD [(Einmalgef\212\247e \()-21(ca. 20 ml)-21(\) zu ver)-21(wenden!)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 56.8329 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Fixierung)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 48.1655 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Um optimale immunhistochemische Ergebnisse zu er)Tj 23.39575 0.00001 TD (-)Tj -23.39575 -1.1154 TD (zielen, wird empfohlen, w\212hrend des gesamten Einbett-)Tj 0 -1.11538 TD [(vorganges bei 4)-169-31(C zu arbeiten und kurze Fixationszeiten)]TJ T* [(anzustreben. M\232glichst kleinste G)-10(ewebest\237cke \()104(1)-169(mm)]TJ 0 -1.11539 TD [(Dicke\) in 2 %-igem P)21(araformaldehyd in Phosphatpuffer)]TJ ET 0 0 0 0 k 212.598 11.339 161.575 428.031 re f BT /F1 1 Tf 6.5 0 0 6.5 212.5984 433.0159 Tm 0 0 0 1 k 0.0003 Tc (p)Tj 0.58325 -0.00001 TD (H)Tj 1.0215 0 Td 0 Tc [(7)125(,4 bei 4)-169-31(C 3)-169-169(4 Stunden fixieren. Anschlie\247end 1)104(2)]TJ -1.60475 -1.11538 TD 0.0001 Tc (S)Tj 0.62508 -0.00001 TD 0 Tc [(tunden \(\237ber Nacht)-21(\) in Phosphatpuffer pH 7)125(,4 mit ei-)]TJ -0.62508 -1.11538 TD [(nem Zusatz von 6,8 %-iger Saccharose bei 4)-169-31(C nach-)]TJ T* (behandeln.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 401.1813 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Entw\212sserung)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 392.514 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Das G)-10(ewebe in kaltem Aceton 1)104(0)-1(0)-169(% mindestens )]TJ 0 -1.11538 TD 0.0001 Tc (1)Tj 0.85413 0 Td 0 Tc [(Stunde bei 4)-169-31(C dehydrieren. W)31(\212hrend der ersten)]TJ -0.85413 -1.11538 TD (Minuten so h\212ufig wechseln, bis das Aceton klar bleibt.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 367.9293 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Infiltration)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 359.262 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Herstellung der Infiltrationsl\232sung:)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 351.5947 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 350.5947 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(echnovit 8)83(1)103(00 Basisl\232sung 1)104(00 ml)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 344.3447 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 343.3447 Tm 0 Tr 0 0 0 1 k 0.0001 Tc (1)Tj 0.85419 0 Td 0 Tc 0 Tw [(Beutel, 0,6 g + T)104(echnovit 8)83(1)104(00 H\212r)-21(ter 1 )]TJ -1.71958 -1.33343 TD (In einem sauberen, sp\237lmittelfreien P)Tj 16.20777 0 TD [(E)-10(- oder G)-10(lasgef\212\247)]TJ -16.20777 -1.11539 TD [(l\232sen und dann in 4)-169-31(C stellen. Die Infiltrationsl\232sung ist)]TJ 0 -1.11538 TD (bei )Tj 1.604 0.00001 TD [(4)-169-32(C verschlossen max)1(i)-1(mal 4 W)32(ochen stabil. P)21(robe)]TJ -1.604 -1.11539 TD [(aus dem Aceton direk)-16(t in die vorgek\237hlte Infiltrations-)]TJ 0 -1.11538 TD (l\232sung \237ber)Tj 5.25 0.00001 TD (f\237hren. Darin bleiben die P)Tj 11.62378 0.00001 TD (roben )Tj 2.854 0.00001 TD (6)Tj 0.73124 0 TD 0.112 Tc [216(0)]TJ -20.45903 -1.1154 TD 0 Tc [(Stunden bei 4)-169-31(C)-1(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 288.3427 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(olymerisation)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 279.6754 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Herstellung der P)21(olymerisationsl\232sung:)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 272.0081 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 271.0081 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Infiltrationsl\232sung, 4)-169-31(C 1)104(5 ml)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 264.7581 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 263.7581 Tm 0 Tr 0 0 0 1 k 0.0004 Tc (+)Tj 0.85475 0 Td 0 Tc 0 Tw [(T)104(echnovit 8)84(1)103(00 H\212r)-21(ter 2, gek\237hlt 0,5 ml)]TJ -1.72014 -1.33344 TD (Mit handels\237blichen Pipettierhilfen abmessen und im)Tj 0 -1.11538 TD (P)Tj 0.66675 0 TD (E)Tj 0.61426 0 TD (-)Tj 0.66707 0 Td (oder G)Tj 3.05119 0 TD [(lasgef\212\247 gut mischen. Danach die infiltrier)-21(t)1(e)]TJ -4.99928 -1.1154 TD (P)Tj 0.62475 0 TD [(robe ca. 5 Minuten vorsichtig im geschlossenen G)-10<65668AA7>]TJ -0.62475 -1.11539 TD (zusammen mischen.)Tj 0 -1.33344 TD [(Die F)21(arbe der P)21(olymerisationsl\232sung ver\212nder)-21(t sich)]TJ 0 -1.11538 TD (zuerst gelblich-gr\237nlich, wird aber farblos nach der)Tj 0 -1.11539 TD [(Aush\212r)-21(tung.)]TJ 0 -1.33343 TD (Mit einer Einmalpipette die Mulden der Histoform voll-)Tj 0 -1.11538 TD (st\212ndig f\237llen, das G)Tj 8.96801 0 TD (ewebe darin orientieren und sofor)Tj 14.81177 0.00001 TD (t)Tj -23.77978 -1.1154 TD (mit der transparenten P)Tj 10.4165 0.00001 TD [(E)-10(-)-10(F)21(olie abdecken. Um die Mulde)]TJ -10.4165 -1.11539 TD [(luftdicht zu verschlie\247en, k\232nnen auch mehrere F)21(olien)]TJ 0 -1.11538 TD (f\237r eine Mulde benutz)Tj 9.59912 0.00001 TD 0.0001 Tc (t)Tj 0.58411 0 Td 0 Tc (werden. Blasen nicht heraus)Tj 12.4364 0 TD (-)Tj -22.61963 -1.1154 TD [(dr\237cken, sondern P)21(olymerisationsl\232sung nachlegen und)]TJ 0 -1.11538 TD [(neue F)21(olie auflegen. W)31(\212hrend der P)21(olymerisation \()-21(min)1(-)]TJ T* [(destens 3 Stunden)-21(\) muss die Einbettform auf einer)]TJ T* [(K)10(\237hlplatte oder d\237nnen Eisschicht bei 4)-169-31(C stehen. F)21(orm)]TJ 0 -1.11539 TD [(und P)21(roben nicht mit F)21(euchtigkeit in K)16(ontak)-16(t bringen.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 126.1714 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Aufblock)Tj 4.14551 0 TD (en und Archivieren)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 117.5041 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Nach der H\212r)Tj 5.93726 0.00001 TD (tung die F)Tj 4.39575 0 TD (olien bei Raumtemperatur mit )Tj -10.33301 -1.11539 TD [(einer Pinzette entfernen. Um die ausgeh\212r)-21(teten P)21(roben)]TJ T* [(aus der T)104(eflonform entnehmen zu k\232nnen, werden diese)]TJ 0 -1.11538 TD (mit Histobloc)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 4.5 0 0 4.5 250.2448 97.7541 Tm 0 Tr 0 0 0 1 k 0 Tc Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 255.8908 95.7541 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(und T)104(echnovit)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 4.5 0 0 4.5 295.8382 97.7541 Tm 0 Tr 0 0 0 1 k 0 Tc Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 301.4842 95.7541 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(3040 aufgeblock)-16(t. Nicht)]TJ -13.67474 -1.11538 TD [(sofor)-21(t ben\232tigte Bl\232cke \()-21(f\237r die Immunhistochemie\) in)]TJ T* (Plastikbeuteln oder \212hnlichem k\237hl lagern.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 71.1695 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(W)31(eiter)-9(verarbeitung)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 62.5021 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Beste S)-10(chneideergebnisse erzielt man mit einem Rota-)]TJ 0 -1.11538 TD [(tionsmikrotom, mit dem T)104(echnovit Histoblade in K)16(o)-1(m-)]TJ T* [(bination mit dem K)10(ulzer Messerhalter oder einem Har)-21(t-)]TJ T* [(metallmesser \(G)-10(las-)-10(Diamantmesser)-21(\). Die Bl\232cke fest im)]TJ T* (Backenklemmsystem am Mikrotom einspannen. Die)Tj 0 -1.11539 TD [(S)-10(chnitte mit einer Pinzette trocken abnehmen und auf)]TJ 0 -1.11538 TD [(einem Streckbad \()-21(Aqua dest.\) aufbringen. Direk)-16(t auf be-)]TJ 26.60206 63.69443 TD (s)Tj 0.479 -0.00001 TD [(chichtete Objek)-16(ttr\212ger aufziehen und )]TJ -0.47899 -1.11538 TD 0.0001 Tc (2)Tj 0.85423 -0.00001 TD 0 Tc [(Stunden oder l\212nger bei 3)42(7 \241)-31(C trocknen. Nicht sofor)-21(t)]TJ -0.85423 -1.11538 TD [(ben\232tigte S)-10(chnitte \()-21(f\237r die Immunhistochemie\) bei)]TJ T* [(Raumtemperatur trocknen und maximal 5 T)104(age bei 4)-169-31(C)]TJ T* (lagern.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5118 393.9313 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Objekttr\212gerbeschichtung)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 385.5118 385.264 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Zum Beispiel Objek)-16(ttr\212ger 1)104(5 Minuten in eine L\232sung)]TJ 0 -1.11538 TD [(von 0,5)-169(% Alcianblau \(8GXL Sigma)-21(\) bei 65)-169-31(C tauchen)]TJ 0 -1.11539 TD [(oder den Objek)-16(ttr\212ger mit 0,)104(1)-113(% P)21(oly-)-10(L)42(-)-10(Lysine fl\237ssig)]TJ 0 -1.11538 TD [(\(Sigma)-21(\) beschichten. Alle handels\237blichen beschichte-)]TJ T* [(ten Objek)-16(ttr\212ger k\232nnen benutz)-16(t werden. Die S)-10(chnitte)]TJ T* (m\237ssen mindestens 2 Stunden bei 3)Tj 16.08324 0 TD [(7)-169-32(C getrocknet)]TJ -16.08324 -1.11539 TD [(sein. Die nichtentplasteten S)-10(chnitte direk)-16(t in die F)21(arb-)]TJ 0 -1.11538 TD [(l\232sung bringen oder mit der enz)-16(ymatischen V)52(orbehand-)]TJ T* (lung beginnen.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5118 317.1793 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Ein Herausl\232sen des K)10(unststoffes vor der F\212rbung)]TJ 0 -1.11539 TD [(oder Reaktion ist nicht m\232glich)-21(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 8.5 0 0 8.5 385.5118 206.8427 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Gefahrenhinweise/)Tj 8.79126 0.00001 TD (Sicherheitsratschl\212ge)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5118 196.7581 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Bitte beachten Sie die Hinweise auf den P)21(roduktver-)]TJ 0 -1.11538 TD [(packungen und Sicherheitsdatenbl\212ttern)-21(.)]TJ T* (www)Tj 2.33324 0 TD (.kulzer-technik)Tj 6.95826 0.00001 TD (.de)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 385.5118 173.5908 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Unsere anwendungstechnische Beratung in W)31(o)-1(r)-21(t,)]TJ 0 -1.11538 TD [(S)-10(chrift und durch V)52(ersuche er)-21(folgt nach bestem Wissen,)]TJ 0 -1.11539 TD (gilt jedoch nur als unverbindlicher Hinweis, auch in)Tj 0 -1.11538 TD (Bezug auf etwaige S)Tj 9.03077 0.00001 TD (chutzrechte Dritter)Tj 8.08275 0.00001 TD (,)Tj 0.50007 0 Td (und befreit Sie)Tj -17.61358 -1.1154 TD [(nicht von der eigenen P)21(r\237fung der von uns geliefer)-21(ten)]TJ 0 -1.11539 TD [(P)21(roduk)-15(te auf ihre Eignung)-1( f\237r die beabsichtigten V)53(er-)]TJ 0 -1.11538 TD (fahren und Zwecke. Anwendung, V)Tj 15.30175 0 TD (er)Tj 0.875 0.00001 TD (wendung und)Tj -16.17675 -1.11539 TD (V)Tj 0.55225 0 TD (erarbeitung der P)Tj 7.64502 0 TD (roduk)Tj 2.55761 0 TD [(te er)-21(folgen au\247erhalb unserer)]TJ -10.75487 -1.11539 TD [(K)16(ontrollm\232glichkeiten und liegen daher ausschlie\247lich )]TJ T* [(in Ihrem V)52(erantwor)-21(tungsbereich. S)-10(elbstverst\212ndlich )]TJ 0 -1.11538 TD (gew\212hrleisten wir die einwandfreie Qualit\212t unserer)Tj (P)' 0.62475 0 TD (roduk)Tj 2.55762 0.00001 TD (te nach Ma\247gabe unserer Allgemeinen V)Tj 17.78101 0 TD (erkaufs-)Tj -20.96338 -1.1154 TD (und Lieferbedingungen.)Tj 0 -1.65384 TD (*)Tj 1.07692 0 TD (Detaillier)Tj 3.72875 0.00001 TD (te Informationen \237ber unsere P)Tj 13.62453 0 TD [(roduk)-16(te finden)]TJ -17.35328 -1.11541 TD (Sie unter www)Tj 6.354 0.00001 TD (.kulzer-technik.de)Tj -7.43092 -1.32385 TD Tj 1.09024 -0.33 TD 0.0001 Tc (=)Tj 0.85412 0 Td 0 Tc [(eingetragenes W)31(arenzeichen, K)10(ulzer GmbH)-21(, Hanau)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 8.5 0 0 8.5 385.5117 292.5092 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(roduktdaten)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5117 277.6746 Tm 0 Tr 0 0 0 1 k 0.021 Tc 0 Tw [(A)22(r)5(t.)21(-N)21(r)63(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 442.2046 277.6746 Tm 0 Tr 0 0 0 1 k 0.001 Tc 0 Tw [(64)43(7)32(090)83(1)105(2)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5117 263.1746 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Bezeichnung)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 442.2046 263.1746 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(echnovit 8)83(1)103(00 K)16(ombipack)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5117 248.6746 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Inhalt)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 442.2046 248.6746 Tm 0 Tr 0 0 0 1 k 0.0002 Tc (1)Tj 0.8544 0 Td (x)Tj 0.7714 0 Td 0 Tc 0 Tw (500 ml Basisl\232sung)Tj -1.6258 -1.11538 TD 0.0001 Tc (5)Tj 0.85428 0 Td (x)Tj 0.77129 0 Td 0 Tc [(0,6 g H\212r)-21(ter 1)]TJ -1.62557 -1.11538 TD 0.0001 Tc (1)Tj 0.85426 0 Td (x)Tj 0.77127 0 Td 0 Tc (30 g H\212r)Tj 3.87494 0 TD (ter 2)Tj -5.50048 -1.11539 TD 0.0001 Tc (1)Tj 0.85415 0 Td (x)Tj 0.77115 0 Td 0 Tc (500 Stk. P)Tj 4.56269 0.00001 TD [(E)-10(-)-10(F)21(olie)]TJ ET 0 J 0 j 10 M []0 d 0.709 w 0 0 0 1 K 385.512 302.93 m 547.087 302.93 l S 385.512 286.937 m 547.087 286.937 l S 0.425 w 385.512 272.764 m 547.087 272.764 l S 385.512 258.59 m 547.087 258.59 l S 0.709 w 385.512 223.228 m 547.087 223.228 l S Q /GS4 gs /GS3 gs q BT /F0 1 Tf 38 0 0 38 39.685 514.9613 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(echnovit)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 15 0 0 15 209.0986 531.3185 Tm 0 Tr 0 0 0 1 k 0 Tc Tj /F3 1 Tf 1 0 0 1 -1000 1566.929 Tm 1 g -0.292 Tc 0 Tw ( )Tj /F4 1 Tf ( )Tj /F5 1 Tf 0 Tc ( )Tj ET Q endstream endobj 9 0 obj << /Name/Fm3 /BBox[-1.20464 -22.09825 15.88904 5.27746] /Subtype/Form /Resources<>>> /Type/XObject /Length 2225 /Matrix[1 0 0 1 0 0] /OPI<> >> /FormType 1 >> stream /GS1 gs q 0 0 1.0176 1 re W n 0 0 0 0 k 1.0002 0.5 m 1.0002 0.2392 0.7802 0.0278 0.5088 0.0278 c 0.2374 0.0278 0.0173 0.2392 0.0173 0.5 c 0.0173 0.7608 0.2374 0.9722 0.5088 0.9722 c 0.7802 0.9722 1.0002 0.7608 1.0002 0.5 c f /GS2 gs 0 J 0 j 4 M []0 d 0.0439 w 0 0 0 1 K 1.0002 0.5 m 1.0002 0.2392 0.7802 0.0278 0.5088 0.0278 c 0.2374 0.0278 0.0173 0.2392 0.0173 0.5 c 0.0173 0.7608 0.2374 0.9722 0.5088 0.9722 c 0.7802 0.9722 1.0002 0.7608 1.0002 0.5 c s 0 0 0 1 k 0.4811 0.2197 m 0.433 0.2197 l 0.4257 0.2812 l 0.4238 0.2781 0.4179 0.2664 0.4116 0.2563 c 0.3796 0.2073 0.3441 0.2057 0.3276 0.2057 c 0.3058 0.2057 0.2581 0.2104 0.2198 0.2827 c 0.2008 0.3193 0.178 0.3807 0.178 0.4904 c 0.178 0.6335 0.2207 0.709 0.2514 0.7424 c 0.2591 0.7502 0.2761 0.7681 0.2994 0.7766 c 0.3164 0.7829 0.3305 0.7829 0.3378 0.7829 c 0.3582 0.7829 0.4014 0.7798 0.4393 0.7167 c 0.4505 0.6989 0.4607 0.6755 0.4665 0.6514 c 0.4719 0.6312 0.4748 0.611 0.4762 0.5892 c 0.4039 0.59 l 0.4029 0.597 0.4014 0.6094 0.3966 0.625 c 0.3903 0.6444 0.3737 0.6817 0.3368 0.6817 c 0.313 0.6817 0.2989 0.667 0.2917 0.6576 c 0.2615 0.6203 0.2538 0.5495 0.2538 0.4927 c 0.2538 0.4383 0.2601 0.3753 0.2863 0.3356 c 0.3062 0.3045 0.3286 0.303 0.3388 0.303 c 0.3446 0.303 0.3587 0.303 0.3728 0.3146 c 0.399 0.3356 0.4126 0.3838 0.4131 0.4282 c 0.3427 0.4282 l 0.3427 0.5169 l 0.4811 0.5169 l f 0.629 0.3154 m 0.6951 0.3154 l 0.696 0.3154 0.7203 0.3146 0.7339 0.3216 c 0.7446 0.3278 0.7611 0.345 0.7611 0.3901 c 0.7611 0.3971 0.7611 0.4173 0.7529 0.436 c 0.7393 0.4686 0.7091 0.464 0.6892 0.4647 c 0.629 0.4647 l 0.629 0.3154 l h 0.629 0.5464 m 0.6936 0.5464 l 0.7106 0.548 0.7281 0.548 0.7398 0.5713 c 0.7427 0.5775 0.7475 0.5907 0.7475 0.6102 c 0.7475 0.6172 0.747 0.6367 0.7398 0.6522 c 0.7271 0.6787 0.7038 0.6755 0.6858 0.6755 c 0.629 0.6755 l 0.629 0.5464 l h 0.6907 0.7689 m 0.7305 0.7689 0.7747 0.772 0.8024 0.7152 c 0.8184 0.6825 0.8204 0.646 0.8204 0.632 c 0.8204 0.6156 0.8184 0.5775 0.7985 0.5456 c 0.7913 0.5332 0.783 0.5254 0.7737 0.5176 c 0.7951 0.5067 0.8364 0.4779 0.8364 0.3823 c 0.8364 0.3722 0.8364 0.3551 0.8316 0.3317 c 0.8267 0.306 0.8121 0.2555 0.7713 0.2322 c 0.7514 0.2205 0.7407 0.2205 0.7033 0.2197 c 0.5557 0.2197 l 0.5557 0.7689 l f Q endstream endobj 10 0 obj << /Name/Fm2 /BBox[0 0 566.92896 566.92896] /Subtype/Form /Resources<>/ProcSet[/PDF]/ExtGState<>>> /Type/XObject /Length 57 /Matrix[1 0 0 1 0 0] /OPI<> >> /FormType 1 >> stream /GS1 gs q 33.166 0 0 20.7092 39.953 457.637 cm /Fm3 Do Q endstream endobj 11 0 obj << /BitsPerSample 8 /Domain[0 1] /Size[255] /FunctionType 0 /Range[0 1 0 1 0 1 0 1] /Length 1020 >> stream                      !""#$$%&&' ( (!)!*"*#+#,$,$ -% .%!.&!/&"0'"0'# 1(# 2(#!2)$!3*$!4*%"4+%"5+,,'$7-'$8-($8.(%9.)%:/)&:/*&;0*'<1+'<1+(=2,(>2,(>3,)?3-)@4-*@4.*A5.+B5/+B6/+C60,D70,D81-E81-F92.F92.G:3.G:3/H;4/I;40I<50J<51K=51K>62L>62M?72M?73N@83O@84OA94PA95QB:5QB:5RC;6SC;6SD<7TE<7UE=8UF=8VF>8WG>9WG>9XH?:YH?:YI@;ZI@;[JA;[JA<\KB<]LB=]LC=^MC>_MD>_ND?`NE?aOE?aOF@bPF@cPGAcQGAdQGBeRHBeSHBfSICgTICgTJDhUJDiUKEiVKEjVLEkWLFkWMFlXMGmXNGmYNHnZOHoZOIo[PIp[PIq\PJq\QJr]QKs]RKs^RLt^SLu_SLu_TMv`TMwaUNwaUNxbVOxbVOycWOzcWPzdXP{dXQ|eYQ|eYR}fYR~gZS~gZSh[Sh[Ti\Ti\Uj]Uj]Vk^Vk^Vl_Wl_Wm`Xn`XnaYoaYoaYpbZpbZqc[qc[rd\rd\se\se]tf]uf^ug^vg_vh_wh`wi`xi`xjayjayjbzkbzkc{lc|lc|md}md}ne~ne~ofofpfpgqgqhrhrisisjsjtjtkukulvlvmwmwmxnxn endstream endobj 12 0 obj << /BitsPerSample 8 /Domain[0 1] /Size[2] /FunctionType 0 /Range[0 1 0 1 0 1 0 1] /Length 8 >> stream xn endstream endobj 13 0 obj [/Separation/PANTONE#207540#20C/DeviceCMYK 12 0 R] endobj 14 0 obj << /Name/Fm5 /BBox[-2.87027 -1.95735 1.31524 10.07274] /Subtype/Form /Resources<>/ProcSet[/PDF]/ExtGState<>>> /Type/XObject /Length 6922 /Matrix[1 0 0 1 0 0] /OPI<> >> /FormType 1 >> stream /GS1 gs q -0.024 0 1.024 1 re W n /CS0 cs 1 scn -0.024 0 0.0035 1 re f 0.3424 0.7231 m 0.3424 0.5774 0.3424 0.4991 v 0.3424 0.4655 0.3427 0.4254 0.3394 0.3998 c 0.3352 0.3682 0.3277 0.3358 0.3038 0.3358 c 0.3038 0.3358 l 0.3038 0.3361 l 0.3038 0.3361 0.3038 0.3358 0.3038 0.3358 c 0.3038 0.3358 0.3038 0.3358 0.3038 0.3358 c 0.3038 0.3358 l 0.2811 0.3358 0.2699 0.3688 0.2666 0.4134 c 0.2647 0.4391 0.2651 0.4655 0.2651 0.4991 c 0.2651 0.5774 0.2651 0.7231 y 0.2349 0.7231 l 0.2349 0.4462 l 0.2349 0.3457 0.2568 0.2622 0.3038 0.2622 c 0.3038 0.2622 0.3038 0.2622 0.3038 0.2622 c 0.3038 0.2622 0.3038 0.2622 0.3038 0.2622 c 0.3508 0.2622 0.3727 0.3478 0.3727 0.4462 c 0.3727 0.7231 l f 0.6848 0.2666 m 0.5494 0.2666 l 0.5494 0.3404 l 0.6475 0.65 l 0.5518 0.65 l 0.5518 0.7231 l 0.6848 0.7231 l 0.6848 0.6538 l 0.5884 0.3397 l 0.6848 0.3397 l f 0.1155 0.0291 m 0.1155 0.1283 l 0.1154 0.1283 l 0.1048 0.0291 l 0.1016 0.0291 l 0.0909 0.1283 l 0.0908 0.1283 l 0.0908 0.0291 l 0.087 0.0291 l 0.087 0.1376 l 0.0935 0.1376 l 0.1031 0.0498 l 0.1032 0.0498 l 0.1127 0.1376 l 0.1193 0.1376 l 0.1193 0.0291 l f 0.1342 0.0291 0.0042 0.1085 re f 0.1643 0.1274 m 0.1643 0.0291 l 0.1601 0.0291 l 0.1601 0.1274 l 0.1488 0.1274 l 0.1488 0.1376 l 0.1756 0.1376 l 0.1756 0.1274 l f 0.2028 0.11 m 0.2018 0.1205 0.1989 0.1289 0.194 0.1289 c 0.1896 0.1289 0.187 0.1229 0.1869 0.1121 c 0.1868 0.0883 0.2082 0.0943 0.2082 0.0594 c 0.2082 0.0437 0.2051 0.0276 0.1955 0.0276 c 0.1872 0.0276 0.1839 0.0429 0.182 0.0558 c 0.1858 0.0598 l 0.1873 0.0483 0.1901 0.0378 0.1957 0.0378 c 0.2012 0.0378 0.204 0.0462 0.204 0.0573 c 0.204 0.085 0.1827 0.0769 0.1827 0.1111 c 0.1827 0.1268 0.1873 0.1391 0.1941 0.1391 c 0.2 0.1391 0.2046 0.1302 0.2065 0.1124 c f 0.2238 0.1376 m 0.2238 0.0609 l 0.2238 0.0483 0.2269 0.0378 0.2328 0.0378 c 0.2387 0.0378 0.2419 0.0483 0.2419 0.0609 c 0.2419 0.1376 l 0.2461 0.1376 l 0.2461 0.0618 l 0.2461 0.0402 0.2406 0.0276 0.2328 0.0276 c 0.225 0.0276 0.2196 0.0402 0.2196 0.0618 c 0.2196 0.1376 l f 0.2609 0.0291 0.0042 0.1085 re f 0.3229 0.0615 m 0.3212 0.0414 0.3173 0.0276 0.3096 0.0276 c 0.3011 0.0276 0.2952 0.0453 0.2952 0.0833 c 0.2952 0.1187 0.3005 0.1391 0.3096 0.1391 c 0.3166 0.1391 0.3207 0.1274 0.3224 0.1075 c 0.3185 0.1042 l 0.3171 0.1196 0.3147 0.1289 0.3091 0.1289 c 0.3036 0.1289 0.2994 0.1145 0.2994 0.0833 c 0.2994 0.0519 0.3036 0.0378 0.3099 0.0378 c 0.3157 0.0378 0.318 0.0504 0.3191 0.0636 c f 0.3597 0.1376 m 0.3597 0.0291 l 0.3555 0.0291 l 0.3555 0.0808 l 0.3374 0.0808 l 0.3374 0.0291 l 0.3332 0.0291 l 0.3332 0.1376 l 0.3374 0.1376 l 0.3374 0.091 l 0.3555 0.091 l 0.3555 0.1376 l f 0.3963 0.1376 m 0.3963 0.1274 l 0.3778 0.1274 l 0.3778 0.091 l 0.3909 0.091 l 0.3909 0.0808 l 0.3778 0.0808 l 0.3778 0.0393 l 0.3973 0.0393 l 0.3973 0.0291 l 0.3736 0.0291 l 0.3736 0.1376 l f 0.4367 0.0291 m 0.4367 0.1283 l 0.4366 0.1283 l 0.426 0.0291 l 0.4227 0.0291 l 0.4121 0.1283 l 0.412 0.1283 l 0.412 0.0291 l 0.4082 0.0291 l 0.4082 0.1376 l 0.4147 0.1376 l 0.4243 0.0498 l 0.4244 0.0498 l 0.4339 0.1376 l 0.4405 0.1376 l 0.4405 0.0291 l f 0.4554 0.0291 0.0042 0.1085 re f 0.4987 0.0615 m 0.4971 0.0414 0.4932 0.0276 0.4855 0.0276 c 0.477 0.0276 0.471 0.0453 0.471 0.0833 c 0.471 0.1187 0.4763 0.1391 0.4855 0.1391 c 0.4925 0.1391 0.4965 0.1274 0.4982 0.1075 c 0.4943 0.1042 l 0.493 0.1196 0.4906 0.1289 0.4849 0.1289 c 0.4795 0.1289 0.4752 0.1145 0.4752 0.0833 c 0.4752 0.0519 0.4795 0.0378 0.4858 0.0378 c 0.4916 0.0378 0.4938 0.0504 0.495 0.0636 c f 0.5137 0.0733 m 0.5251 0.0733 l 0.5197 0.1292 l 0.5191 0.1292 l 0.5137 0.0733 l h 0.5047 0.0291 m 0.5163 0.1376 l 0.5225 0.1376 l 0.5341 0.0291 l 0.5297 0.0291 l 0.5262 0.0631 l 0.5126 0.0631 l 0.5091 0.0291 l f 0.5437 0.0291 m 0.5437 0.1376 l 0.5478 0.1376 l 0.5478 0.0393 l 0.5657 0.0393 l 0.5657 0.0291 l f 0.5932 0.11 m 0.5922 0.1205 0.5892 0.1289 0.5844 0.1289 c 0.58 0.1289 0.5774 0.1229 0.5773 0.1121 c 0.5772 0.0883 0.5985 0.0943 0.5985 0.0594 c 0.5985 0.0437 0.5955 0.0276 0.5858 0.0276 c 0.5776 0.0276 0.5743 0.0429 0.5723 0.0558 c 0.5762 0.0598 l 0.5777 0.0483 0.5805 0.0378 0.586 0.0378 c 0.5916 0.0378 0.5944 0.0462 0.5944 0.0573 c 0.5944 0.085 0.5731 0.0769 0.5731 0.1111 c 0.5731 0.1268 0.5777 0.1391 0.5845 0.1391 c 0.5903 0.1391 0.5949 0.1302 0.5969 0.1124 c f 0.6536 0.0812 m 0.6536 0.0291 l 0.6501 0.0291 l 0.6501 0.039 l 0.65 0.039 l 0.6481 0.0313 0.6443 0.0276 0.6402 0.0276 c 0.6316 0.0276 0.6254 0.0453 0.6254 0.0833 c 0.6254 0.1214 0.6316 0.1391 0.6402 0.1391 c 0.6465 0.1391 0.6514 0.1304 0.6538 0.1073 c 0.6498 0.105 l 0.6479 0.1223 0.6448 0.1289 0.6404 0.1289 c 0.6348 0.1289 0.6296 0.1181 0.6296 0.0833 c 0.6296 0.0486 0.6348 0.0378 0.6404 0.0378 c 0.6465 0.0378 0.6494 0.0486 0.6494 0.0618 c 0.6494 0.071 l 0.6409 0.071 l 0.6409 0.0812 l f 0.6698 0.0886 m 0.6774 0.0886 l 0.6832 0.0886 0.6862 0.0948 0.6862 0.1084 c 0.6862 0.123 0.6828 0.1274 0.6774 0.1274 c 0.6698 0.1274 l 0.6698 0.0886 l h 0.6657 0.0291 m 0.6657 0.1376 l 0.6778 0.1376 l 0.6859 0.1376 0.6904 0.1271 0.6904 0.109 c 0.6904 0.0937 0.6873 0.085 0.6829 0.0808 c 0.6909 0.0291 l 0.6863 0.0291 l 0.6787 0.0784 l 0.6698 0.0784 l 0.6698 0.0291 l f 0.7154 0.0378 m 0.722 0.0378 0.7259 0.0525 0.7259 0.0833 c 0.7259 0.1142 0.722 0.1289 0.7154 0.1289 c 0.7087 0.1289 0.7048 0.1142 0.7048 0.0833 c 0.7048 0.0525 0.7087 0.0378 0.7154 0.0378 c h 0.7154 0.1391 m 0.7246 0.1391 0.7301 0.1196 0.7301 0.0833 c 0.7301 0.0471 0.7246 0.0276 0.7154 0.0276 c 0.7061 0.0276 0.7006 0.0471 0.7006 0.0833 c 0.7006 0.1196 0.7061 0.1391 0.7154 0.1391 c f 0.7453 0.1376 m 0.7453 0.0609 l 0.7453 0.0483 0.7484 0.0378 0.7543 0.0378 c 0.7603 0.0378 0.7634 0.0483 0.7634 0.0609 c 0.7634 0.1376 l 0.7676 0.1376 l 0.7676 0.0618 l 0.7676 0.0402 0.7621 0.0276 0.7543 0.0276 c 0.7466 0.0276 0.7411 0.0402 0.7411 0.0618 c 0.7411 0.1376 l f 0.7853 0.0877 m 0.7924 0.0877 l 0.7993 0.0877 0.8017 0.0946 0.8017 0.1075 c 0.8017 0.1205 0.799 0.1274 0.7933 0.1274 c 0.7853 0.1274 l 0.7853 0.0877 l h 0.7811 0.0291 m 0.7811 0.1376 l 0.7943 0.1376 l 0.8023 0.1376 0.8058 0.1244 0.8058 0.1073 c 0.8058 0.0892 0.8015 0.0775 0.792 0.0775 c 0.7853 0.0775 l 0.7853 0.0291 l f 0.7379 0.3414 m 0.7379 0.4532 l 0.831 0.4532 l 0.831 0.5228 l 0.7379 0.5228 l 0.7379 0.6485 l 0.8384 0.6485 l 0.8384 0.7231 l 0.7082 0.7231 l 0.7082 0.2666 l 0.7379 0.2666 l 0.8406 0.2666 l 0.8406 0.3414 l f 0.9651 0.4696 m 0.9844 0.4854 0.9943 0.5308 0.9941 0.5869 c 0.9939 0.6619 0.9794 0.7231 0.9434 0.7231 c 0.8646 0.7231 l 0.8646 0.6528 l 0.9264 0.6528 l 0.9382 0.6526 l 0.9557 0.6526 0.9638 0.6297 0.9638 0.5869 c 0.9638 0.544 0.9566 0.5229 0.9329 0.5229 c 0.9264 0.5229 l 0.8646 0.5227 l 0.8646 0.2666 l 0.8949 0.2666 l 0.8949 0.4533 l 0.9364 0.4533 l 0.9664 0.2666 l 1 0.2666 l f 0.0871 0.2666 0.0303 0.4565 re f 0.4009 0.2666 m 0.4009 0.7231 l 0.4312 0.7231 l 0.4312 0.3397 l 0.5282 0.3397 l 0.5282 0.2666 l f 0.1614 0.4984 m 0.2138 0.7231 l 0.1813 0.7231 l 0.1344 0.5188 l 0.1344 0.4783 l 0.1902 0.2666 l 0.2238 0.2666 l f Q endstream endobj 15 0 obj << /Name/Fm4 /BBox[0 0 566.92896 566.92896] /Subtype/Form /Resources<>/ProcSet[/PDF]/ExtGState<>>> /Type/XObject /Length 62 /Matrix[1 0 0 1 0 0] /OPI<> >> /FormType 1 >> stream /GS1 gs q 135.45052 0 0 47.12589 388.7795 92.242 cm /Fm5 Do Q endstream endobj 16 0 obj << /Length 18011 >> stream /GS0 gs q 0 0.929 566 566 re W n 1 g 0 566.929 0 0 re f Q /GS1 gs /GS3 gs q BT /F0 1 Tf 11.75 0 0 11.75 78.6614 463.3376 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(User instruction T)104(echnovit 8)73(1)94(0)-1(0)]TJ ET 0 0 0 0.1 k -8.504 -8.504 36.85 583.937 re f 0 0 0 0.7 k -8.504 491.811 583.937 4.252 re f 0 0 0 0 k 39.685 10.261 161.575 431.944 re f Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 435.8506 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(S)-10(ections specifically for immunohistochemistr)-21(y)]TJ 0 -2.23077 TD (T)Tj 0.45851 -0.00001 TD [(echnovit 8)83(1)104(00 is a H)-42(E)-42(MA-based plastic-embedding )]TJ -0.45851 -1.11537 TD [(system for studies with light microscopy)42(. It is suitable for)]TJ 0 -1.11539 TD [(embedding all tissues in medicine, zoology and b)-10(otany)42(.)]TJ 0 -1.11538 TD [(S)-10(ections of decalcified or briefly decalcified illiac crest)]TJ T* (biopsies and implanted biomaterials can be used for)Tj 0 -1.11539 TD (more than just histological staining; they can also be)Tj 0 -1.11538 TD [(used for enz)-16(yme chemistr)-21(y and immunohistochemistr)-21(y)42(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 367.7659 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Material proper)-16(ties)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 359.0986 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)103(echnovit 8)84(1)103(00 is a combination of a practically odour-)]TJ 0 -1.11538 TD [(less plasticizer and a hydrophillic plastic. T)104(echnovit 8)83(1)104(0)-1(0)]TJ 0 -1.11539 TD (was specifically developed for cold polymerisation)Tj 0 -1.11538 TD [(\(+4 \241)-31(C\). )]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 327.264 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (While hardening the embedding form must be her)Tj 23.31275 0 TD (-)Tj -23.31275 -1.11538 TD (metically sealed because the polymerisation system)Tj T* (is oxygen-sensitive.)Tj 0 -1.98758 TD [(Over)-10(view of the benefits)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 292.1774 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 291.1774 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Reproducibility and reliability of the embedding due to)Tj 0 -1.11539 TD (the constant, documented quality controls of the )Tj T* (individual components)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 269.01 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 268.01 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(L)10(ow polymerisation temperature of 1)104(0 \241)-31(C to 0 \241)-32(C due)]TJ 0 -1.11538 TD (to the special catalyst system and the T)Tj 17.18775 0 TD (eflon forms)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 253.0927 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 252.0927 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Uniform hardening of the block, thus uniform and )Tj 0 -1.11539 TD (thinnest possible sections)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 237.1754 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 236.1754 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(L)10(ow shrinkage ar)-21(tefacts, thus excellent tissue morpho-)]TJ 0 -1.11539 TD 0.001 Tc (logy)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 221.2581 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 220.2581 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Routine staining, enz)-16(yme detection and immunohisto-)]TJ 0 -1.11538 TD [(chemistr)-21(y possible)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 205.3407 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 204.3407 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Haemotological illiac crest biopsies do not need to be)Tj 0 -1.11538 TD (decalcified)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 39.685 189.4234 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 45.31 188.4234 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(L)10(ow toxicity due to special combination of plas)-1(ticizer)]TJ 0 -1.11538 TD (and catalyst system)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 171.0888 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Application)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 162.4214 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62477 0 TD 0 Tw (repare T)Tj 3.56225 0.00001 TD (echnovit 8)Tj 4.45849 0.00001 TD [(1)103(00 in accordance with)1( the step-by)]TJ -8.64551 -1.1154 TD (step instructions. Place the fixated and dehydrated )Tj 0 -1.11538 TD (specimens in the infiltration solution. A low temperature)Tj 0 -1.11539 TD (and agitation of the specimens is beneficial during the)Tj 0 -1.11538 TD (entire embedding process.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 123.3368 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)10(he following instructions for fixation and dehydra-)]TJ 0 -1.11538 TD (tion are not necessarily required)Tj 15.16675 0 TD (. T)Tj 1.021 0.00001 TD (echnovit can also)Tj -16.18775 -1.1154 TD (be infiltrated and polymerised after other pre-treat)Tj 23.64649 0.00001 TD (-)Tj -23.64649 -1.11539 TD [(ment)-21(. )]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 91.5022 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Air)Tj 1.20826 0.00001 TD (tight glass or P)Tj 6.60449 0.00001 TD (E disposable containers \()Tj 11.04151 0.00001 TD (approx. 20)Tj -18.85426 -1.1154 TD (ml)Tj 1.08351 0 TD (\) must be used for the entire process!)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 39.685 74.1675 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Fixation)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 39.685 65.5002 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (In order to achieve optimal immunohistochemical re)Tj 22.74928 0.00001 TD (-)Tj -22.74928 -1.11539 TD [(sults, it is recommended to work at 4 \241)-31(C throughout the)]TJ 0 -1.11538 TD (entire embedding process and to aim for shor)Tj 20.16626 0.00001 TD (t fixation)Tj -20.16626 -1.11539 TD [(times. Fix the smallest possible pieces of tissue \()104(1)-169(mm)]TJ T* [(thickness)-21(\) in 2 % paraformaldehyde in phosphate buffer)]TJ 0 -1.11538 TD [(pH 7)125(.4 at 4 \241)-31(C for 3)-169-112(4 hours. Subsequently)42(, retreat for)]TJ T* [(1)103(2 hours \()-20(overnight)-21(\) in phosphate buffer pH 7)125(.4 with an)]TJ 0 -1.11539 TD [(additional 6.8 % sucrose at 4 \241)-31(C)-1(.)]TJ ET 0 0 0 0 k 212.598 10.173 161.575 432.032 re f Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 435.8141 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Dehydration)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 427.1468 Tm 0 Tr 0 0 0 1 k 0 Tc (D)Tj 0.68802 -0.00001 TD 0 Tw [(ehydrate the tissue in cold acetone 1)104(00 % for at least)]TJ -0.68802 -1.11538 TD 0.0001 Tc (o)Tj 0.56211 -0.00001 TD 0 Tc (ne hour at 4 )Tj 5.64638 0 TD [-31(C. Change as often as possible in the first)]TJ -6.20849 -1.11538 TD [(minutes until the acetone remains clear)42(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 402.5621 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Infiltration)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 393.8948 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Making the infiltration solution:)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 386.2275 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 385.2275 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(echnovit 8)83(1)103(00 basic solution 1)104(00 ml)]TJ ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 378.9775 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 377.9775 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(1 bag 0,6 g + T)104(echnovit 8)83(1)104(00 hardener 1)]TJ -0.86538 -1.33345 TD [(Dissolve in a clean, detergent-free P)-21(E or glass container)]TJ 0 -1.11538 TD [(and then place at 4 \241)-31(C. When sealed, the infiltration )]TJ T* [(solution is stable for a maximum of four weeks at 4 \241)-31(C. )]TJ 0 -1.33343 TD [(T)104(ransfer the specimen directly from the acetone to the)]TJ 0 -1.11538 TD [(pre-cooled infiltration solution. T)10(he specimens remain)]TJ 0 -1.11539 TD [(therein for 6)-169104(0 hours at 4 \241)-31(C)-1(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 321.5582 Tm 0 Tr 0 0 0 1 k 0 Tc (P)Tj 0.62475 0 TD 0 Tw (olymerisation)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 312.8908 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Making the polymerisation solution:)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 305.2235 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 304.2235 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Infi ltrationsolution, 4 )Tj 9.33326 0 TD Tj 0.38525 0 TD (C 1)Tj 1.45849 0.00001 TD (5 ml)Tj ET Q /GS4 gs /GS3 gs q BT /F2 1 Tf 4 0 0 4 212.5984 297.9735 Tm 0 Tr 0 0 0 1 k 0 Tc (x)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 218.2234 296.9735 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(+ T)104(echnovit 8)84(1)103(00 hardener 2, cooled 0,5 ml)]TJ -0.86538 -1.33343 TD (Measure with standard pipetting aids and mix well in a)Tj 0 -1.11538 TD (P)Tj 0.66675 0 TD (E or glass container)Tj 8.66626 0 TD (. T)Tj 1.05249 0 TD (hen, carefully mix the infiltrated)Tj -10.3855 -1.1154 TD (specimen in a sealed container for approx. five minutes. )Tj 0 -1.33343 TD -0.003 Tc [(T)9(he colour of the polymerisation solution changes first to)]TJ 0 -1.11538 TD (yellow-green, but after hardening it becomes colourless. )Tj 0 -1.11539 TD 0 Tc (Completely fill the Histoform cavities with a disposable)Tj 0 -1.11538 TD (pipette, position the tissue therein and immediately )Tj T* [(cover with transparent P)-21(E film. Multiple films can be)]TJ 0 -1.11539 TD [(used for a cavity in order to hermetically seal the cavity)42(.)]TJ T* [(Do not press out bubbles; rather)42(, apply more polymeri-)]TJ 0 -1.11538 TD (sation solution and add new film. During polymerisation)Tj 0 -1.11539 TD [(\()-21(at least 3 hours)-21(\) the embedding form must be placed)]TJ T* [(on a cooling plate or thin layer of ice at 4 \241)-31(C. Do not let)]TJ 0 -1.11538 TD (the form or specimens come into contact with moisture.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 182.5542 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Blocking and archiving)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 173.8869 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Remove the film at room temperature with tweezers on-)Tj 0 -1.11539 TD [(ce hardening is complete. T)10(he specimens are blocked)]TJ 0 -1.11538 TD (with Histobloc)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 4.5 0 0 4.5 253.0885 161.3869 Tm 0 Tr 0 0 0 1 k 0 Tc Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 258.7345 159.3869 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (and T)Tj 2.41675 0 TD (echnovit)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 4.5 0 0 4.5 298.4105 161.3869 Tm 0 Tr 0 0 0 1 k 0 Tc Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 304.0565 159.3869 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (3040 so that they can)Tj -14.07047 -1.11539 TD [(be removed from the T)104(eflon mould. Store blocks that are)]TJ 0 -1.11538 TD [(not needed immediately \()-21(for immunohistochemistr)-21(y)-21(\) at a)]TJ T* (cool temperature in plastic bags or similar)Tj 18.33228 0 TD (.)Tj ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 127.5522 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(rocessing)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 118.8849 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (One obtains the best cutting results with a rotation )Tj 0 -1.11539 TD [(microtome, with the T)104(echnovit Histoblade in combina-)]TJ 0 -1.11538 TD (tion with the K)Tj 6.32373 0 TD (ulzer knife holder or a hard metal knife)Tj -6.32373 -1.11539 TD [(\()-21(glass diamond knife\). T)10(ightly clamp the blocks in the )]TJ T* (totem cam system on the microtome. Dr)Tj 17.70898 0 TD (yly remove the)Tj -17.70898 -1.11538 TD (sections with forceps and place in a bath \()Tj 18.56226 0.00001 TD (Aqua dest.\).)Tj -18.56226 -1.11539 TD (Placed directly on a coated object holder and let dr)Tj 22.47778 0.00001 TD (y )Tj -22.47778 -1.11539 TD [(for 2 hours or moreat 3)42(7 \241)-31(C. Dr)-21(y sections that are not)]TJ T* [(needed immediately \()-21(for immunohistochemistr)-21(y)-21(\) at room)]TJ 0 -1.11538 TD [(temperature at store for a maximum of five days at 4 \241)-31(C)-1(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 212.5984 43.5503 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Object holder coating)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 212.5984 34.883 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(F)21(or example, submerse the object holder in a solution of)]TJ 0 -1.11538 TD [(0.5 % alcian blue \(8GXL Sigma)-21(\) at 65 \241)-31(C for 1)104(5 minutes)]TJ T* [(or liquidly coat the object holder with 0.)104(1 % poly-)-10(L)42(-lysine)]TJ 0 -1.11539 TD [(\(Sigma)-21(\). All standard coated object holders may be)]TJ 26.60206 65.03349 TD [(used. T)10(he sections must dr)-21(y for at least two hours at )]TJ 0 -1.11539 TD (3)Tj 0.52051 0 TD [(7 \241)-31(C. Place the non-deplasticized sections directly in)]TJ -0.52051 -1.11539 TD 0.0001 Tc (t)Tj 0.2921 -0.00001 TD 0 Tc [(he stain solution or star)-21(t with enz)-16(ymatic pre-treatment.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5118 411.2659 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (It is not possible to elute the plastic before staining)Tj 0 -1.11538 TD [(or reaction)-21(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 8.5 0 0 8.5 385.5118 302.3467 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Danger/)-21(Safety advices)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5118 292.262 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Please follow the instructions on the product )Tj 0 -1.11538 TD (packaging and safety data sheets)Tj 15.87549 0 TD (.)Tj -15.87549 -1.11539 TD (www)Tj 2.33324 0 TD [(.kulzer-technik)-21(.com)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 385.5118 269.0947 Tm 0 Tr 0 0 0 1 k -0.003 Tc 0 Tw (Our technical advice, whether verbal, in writing or by way)Tj 0 -1.11538 TD (of trials, is given in good faith but without warranty)Tj 21.80615 0.00001 TD (, and)Tj -21.80615 -1.11539 TD (this also applies where proprietar)Tj 14.44555 0.00001 TD (y rights of third par)Tj 8.27429 0 TD (ties)Tj -22.71984 -1.1154 TD (are involved. It does not release you from the obligation)Tj 0 -1.11538 TD (to test the products supplied by us as and to their suita-)Tj 0 -1.11539 TD [(bility for the intended processes and uses. T)10(he applica)1(-)]TJ 0 -1.11538 TD (tion, use and processing of the products are beyond our)Tj T* [(control, and therefore, entirely your own responsibility)43(.)]TJ 0 -1.11539 TD (Should, in spite of this, liabilty be established for any )Tj T* (damage, it will be limited to the value of the goods )Tj 0 -1.11538 TD (delivered by us and used by you. W)Tj 15.4768 0.00001 TD (e will, of course, )Tj -15.4768 -1.11539 TD (provide consistent quality of our products within the )Tj 0 -1.11538 TD [(scope of our G)-10(eneral Conditions of Sale and Deliver)-20(y)41(.)]TJ 0 -1.65384 TD 0 Tc [(*)-723(F)21(or more information please visit )]TJ 1.07692 -1.11538 TD [(www)42(.kulzer-technik.com)]TJ -1.07692 -1.32384 TD Tj 1.09024 -0.33 TD [(= registered trademark, K)10(ulzer GmbH)-21(, Hanau)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 8.5 0 0 8.5 385.5116 386.4621 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(P)21(roduct data)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5116 371.6275 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(Item No)-21(.)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 442.2044 371.6275 Tm 0 Tr 0 0 0 1 k 0.001 Tc 0 Tw [(64)43(7)31(090)84(1)105(2)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5116 357.1275 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Designation)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 442.2044 357.1275 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)103(echnovi)1(t 8)83(1)103(00 Combipack)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 6.5 0 0 6.5 385.5116 342.6275 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (Quantity)Tj ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 6.5 0 0 6.5 442.2044 342.6275 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw (1 x 500 ml basic solution)Tj 0 -1.11538 TD (5 x 0,6 g hardener 1)Tj T* (1 x 30 g hardener 2)Tj T* [(1 x 500 pcs. P)-21(E films)]TJ ET 0 J 0 j 10 M []0 d 0.709 w 0 0 0 1 K 385.512 396.883 m 547.086 396.883 l S 385.512 380.89 m 547.086 380.89 l S 0.425 w 385.512 366.717 m 547.086 366.717 l S 385.512 352.543 m 547.086 352.543 l S 0.709 w 385.512 317.181 m 547.086 317.181 l S Q /GS4 gs /GS3 gs q BT /F0 1 Tf 7 0 0 7 399.8385 68.271 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(K)-10(U)-43(LZ)-20(E)-42(R GmbH)]TJ 0 -1.31609 TD (L)Tj 0.55249 0.00001 TD (eipziger Stra\247e 2)Tj -0.55249 -1.31609 TD (63450 Hanau)Tj 6.22876 0.00001 TD (, Deutschland)Tj -6.22876 -1.31609 TD (T)Tj 0.47925 0.00001 TD (el)Tj 0.8335 0.00001 TD (.)Tj 0.6584 0.00001 TD (+49 (0) 6)Tj 4.01026 0.00001 TD (1)Tj 0.48925 0.00001 TD [(8)73(1)93(/9689-25)43(7)51(4 o)-20(. 25)42(7)93(1)]TJ -6.47065 -1.31613 TD [(F)20(ax)-349(+49 (0) 6)73(1)93(8)73(1)94(/9689-2964)]TJ 0 -1.31609 TD [(technik)-21(.wehrheim@kulzer-dental)-20(.com)]TJ 0 -1.31608 TD [(www)42(.kulzer-technik)-21(.de)]TJ ET Q /GS4 gs /GS3 gs q BT /F1 1 Tf 0 6 -6 0 561.4479 12.0236 Tm 0 Tr 0 0 0 1 k 0.0005 Tc (6)Tj 0.5625 0 TD (6)Tj 0.5625 0 TD (0)Tj 0.5625 0 TD (6)Tj 0.5625 0 TD (2)Tj 0.5625 0 TD (8)Tj 0.5625 0 TD (0)Tj 0.5625 0 TD (0)Tj 0.5625 0 TD (_)Tj 0.5005 0 TD (1)Tj 0.458 0 TD 0.0001 Tc (1)Tj 0.56209 0 TD 0 Tw ( )Tj 0.29209 0 TD Tj 0.68809 0 TD ( )Tj 0.29209 0 TD (2)Tj 0.56209 0 TD (-)Tj 0.37509 0 TD (s)Tj 0.47909 0 TD (p)Tj 0.58309 0 TD (r)Tj 0.29099 0 TD 0.0005 Tc (.)Tj 0.2085 0 TD (/)Tj 0.396 0 TD 0 Tc (D)Tj 0.7185 0 TD (O)Tj 0.78101 0 TD (G)Tj 0.80199 0 TD 0.0008 Tc (/)Tj 0.35475 0 TD (0)Tj 0.479 0 TD 0 Tc (1)Tj 0.45851 0 TD (.)Tj 0.10425 0 TD (1)Tj 0.45849 0 TD (7)Tj ET /GS4 gs q 37.118 450.216 36 30.965 re W n /Fm2 Do Q Q /GS4 gs /GS3 gs q BT /F0 1 Tf 28 0 0 28 233.8583 514.9016 Tm 0 Tr 0 0 0 1 k -0.073 Tc 0 Tw [(81)20(0)-74(0)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 38 0 0 38 39.685 514.9622 Tm 0 Tr 0 0 0 1 k 0 Tc 0 Tw [(T)104(echnovit)]TJ ET Q /GS4 gs /GS3 gs q BT /F0 1 Tf 15 0 0 15 209.0986 531.3194 Tm 0 Tr 0 0 0 1 k 0 Tc Tj ET 0 J 0 j 10 M []0 d 0.5 w 0 0 0 1 K 400.118 81.967 m 524.842 81.967 l S /GS4 gs q 388.779 90.764 136.827 48.604 re W n /Fm4 Do Q /GS3 gs 385.7519 139.6061 m 385.7519 81.4961 l S Q endstream endobj 17 0 obj << /Nums[0<>] >> endobj 18 0 obj << /BaseEncoding/MacRomanEncoding /Type/Encoding /Differences[32/space 40/parenleft/parenright 43/plus/comma/hyphen/period/slash/zero/one/two/three/four/five/six/seven/eight/nine 64/at/A/B 68/D/E/F/G/H/I 75/K/L/M/N/O/P/Q/R/S/T/U/V/W 90/Z 97/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z 138/adieresis 154/odieresis 167/germandbls/registered] >> endobj 19 0 obj << /Subtype/Type1C /Length 7699 /Length1 7699 >> stream SZTZLV+NeueHelveticaBQ-Bold=Wk   d;Qb(c) COPYRIGHT H. BERTHOLD AG 1992 Alle Rechte vorbehalten.Neue Helvetica 75 BoldNeue Helvetica BQF(FCSBVDITJOGPNU5W.MH8E)& 1ZQ%7#L"X['Y*0K,34/6R2-;  !H$^h.7l  K y  d z  Ax 3k T A^9l.hvy5g0 "%RoMUpn|nv]bU~܋ыԛɻ׋[rw{)vt=2a|nh[ht{L`3*L!<\.텸ԍ<3|#r#p$QMr0&*"%axŸ{ocey5sȄtdut]|Gv "P}vkԋԠŋNj`5vKX]Q V5xmVaBm21QK ̲v! u|BxoOv p3|$]|x{ww[M~Tlibrmke~Ruz{RvYK\uBhHoM-&潰}qTkz}}nuXK}H| -vYZY`iqvmTwijk*ҵC3|"zL32J(tRwYVzKne~ɋܫMdbJͳɶvaHv/HvXUVVue~mm@_fwv|Ou{|Qx4LYy}kjL.oxŅxz~tq}}smYm~|be1ƯneX'lqu܇~x~}$vv !WV @ Hv/XQsbij*mKcsv|w|~vѠv4!VyxxSK]ckgc^p;+4!4+H|""0K'uSwfT'ayŸԍ勬sMG=k wjTXVK]kzqpqqjq ʵv/MLQvkizolsFCD^mvx}~|l]Z9^dvr (/!/8+mmncs|{|d~Hvj/Vi/i*vv 'E1HsL!!4|vnt^jwZSUhy#DZÎNjgrmf}mJ)qN=e|uüoMtyt}ytyYXo~ȈRvu~qwo|~tdHVы³ΉHvW v(-Kcql}zyXnUx#8W)HuvQpP\R2Cmpon >`HqŎŽkmO/f afM*!p(6\st‘ȞuvVv&g&Vm47oV&HF&$vVv!WV V ]S H}^Qx)UWtmUF7q(SlNՈP]Z[sn}mPo~!y'|׋ݛȹ‹Ќ㌯G<Blaqzzrn9p̤vVv0V.&/PV1 /3| WTd|kT-Tlhbjbdwԋldgv}wowXA[rr}[h=kmpG⌠軰}qTq~sfbFJ}]|Gv" .Vw`E'?Ml-{Z]yk@bzƋ֋KV  |`?} MfQ}kn\ؘᬶŠvv//V//V//rB /-VVB /$5vJ14a141jwy|Ev a.*VV.!ˌƍ׋Vg\]T@ uxmkkvob]_K v& ,Hm}|q~~|hS芧Ɉvd]5v; "PI vjՋ݋Ջ񋩋.?Ndg|Ecnwx}yhlzbPf^um{pnWmoU܋ ȲƋ$14a1414ŋT-/VVƌʼnġĠßȔԍ+ XXLtut~P*v5v\ulcSYM rvVv V4445{VCT: +/VV،ߩ̕6_jR09  iwrrzsmgh"ɑasqQiuuoY3vvkv:IQVB!x?lv2Gv><YvVv/iV/V/y{0 0h"BUnEEqh -gpɉʰ5.J]4$bȊs{Z:Ev2M{jxZ:\z]ʋꋛ#í$Nv !W!YwjwtқW H| ""bTdM0K'uSwfT'ayŸԍ勬sMG=k wjTXVK]kzqpqqjq ʵŠvVv-@VW-V-xjW?vX-)VV-twh`__l}}5y{sex⋷qT7myyx> ,ƨt{j{VWvywc&vzv$i|y '+k,kb]~%DLmRwed|rl=ttzvyvq~]Lztw}/e,Zpv@:EѺlm`YTPR`yzߚ܋Hz{ŠvVv)&V)xx1V){{.Kȫ9(%uqysdLU/YX~|akbm"qtt\Xt\F#+37f`nhW@ 21g\qڋ싶k8]Zzcw Z{'߶ܐ&4`<:hyv.. V.<UMWi|o`pĂ=.2>ldVn|hl̴䌝]5v;" LvcFUGuhkWPO@ASsd݋ vc;}NdO9t̄y{0 0 )M_2$_,9?eZA,cn-it‰ǣ.w{uJPkhȋמfKuC{\q:E=3v//V|ZV^,fHB k"a|vaGdkquqz~ѯ®]my|w{uK +)e'l}_Z]J iϐnTt|{njwHH ^h" ŲñЗoxnS"'+Zf*vTUxdzbPGUf{{zz/P`m:yZawH*qtSpVQd"Cq E]swzk{؋ӫhe\pls~dkjvny}vҜSu%/ӊLK_y[dt~tvDcɛʨH eA~&]a}ٌƒՋ|gnp{Ƌv~Mp(P,Tyhgz|{nx~zdrH'яS}v{ivD-dhuyHv38v.7*33tNPH }"EH؋Yoz|zDcL={Xm%]}סĥחe7`dzOdvzz.Hv#immiillHq+C8cwbˈ5ًMJ*d)/Tc/y`b{"OteLJ氾zUxVpCd~eBy†.ӌJKKcMEErì8vvM~~\z􆵄Ž% }x}wdfA}XhvuvoQA*Pa 7c8vvZhbeonm2[Wiu{c%xavwv .Œ y|y|ly}Hv1&Enuu~M:P k+i%;`&ό8ǯĞATuijddymsFRf'4._yz`ձ F^B;m#K3. S$Mg5\yU cyxtuZl$I4Y Z v  vmWl.X9o]lptv/<b*xDfT`me|vwhǦ忰xlmy6;T9  昞   endstream endobj 20 0 obj << /XHeight 901 /Ascent 891 /Type/FontDescriptor /StemV 138 /StemH 0 /CapHeight 706 /FontName/SZTZLV+NeueHelveticaBQ-Bold /Descent -182 /FontBBox[-195 -262 1034 983] /Flags 262176 /ItalicAngle 0 /FontFile3 19 0 R >> endobj 21 0 obj << /FontDescriptor 20 0 R /Encoding 18 0 R /Name/F0 /Type/Font /Subtype/Type1 /FirstChar 32 /BaseFont/SZTZLV+NeueHelveticaBQ-Bold /Widths[292 0 0 0 0 0 0 0 292 292 0 583 250 375 250 396 583 583 583 583 583 583 583 583 583 583 0 0 0 0 0 0 896 688 667 0 708 625 562 708 708 271 0 708 562 875 708 750 646 750 688 646 583 708 625 917 0 0 646 0 0 0 0 0 0 562 604 562 604 562 333 604 583 250 250 562 250 896 583 583 604 604 375 521 354 583 500 792 542 521 521 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 562 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 583 0 0 0 0 0 0 0 0 0 0 0 0 583 833] /LastChar 168 >> endobj 22 0 obj << /BaseEncoding/MacRomanEncoding /Type/Encoding /Differences[32/space/exclam 37/percent 40/parenleft/parenright/asterisk/plus/comma/hyphen/period/slash/zero/one/two/three/four/five/six/seven/eight/nine/colon/semicolon 61/equal 65/A/B/C/D/E/F/G/H/I 75/K/L/M/N/O/P/Q/R/S/T/U/V/W/X 90/Z 95/underscore 97/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v/w/x/y/z 138/adieresis 154/odieresis 159/udieresis 161/degree 167/germandbls/registered 208/endash] >> endobj 23 0 obj << /Subtype/Type1C /Length 8327 /Length1 8327 >> stream WLTLKJ+NeueHelveticaBQ-Light=:_   | `;Rc(c) COPYRIGHT H. BERTHOLD AG 1992 Alle Rechte vorbehalten.Neue Helvetica 45 LightNeue Helvetica BQQ4DIOJUFTQ[MGSE*NVP5W,CZB#)&."L6H(X ;'8 $ 32/1Y%oR0K-97 @SIW!F\QXv Z  \ 7 # [  - Vysb'RSa )q0#:r| 1j_82[vjsZhpWOmCc~~}zKwwzelh\cLPtYKUayOtoɋҋjsrrqpqQfsxn˵ֳvvlcZ|H}YmB_prytywk`H:t*$_؎M~yjgA&qsvyJƊҡ̳㋯Tk}|3vkJvVMVtUsrsy{](/[[mw3vjčƳsgZVÉw^D4^btouPNvv YM%*M-!/M/2XYbyU||}U|`K{*vbI@B(m}^a{mEbʼnԹOtwuv{{mtST_le‹̊ʿЋ֋VCru?|I?_w*Ycvf{nq~Xcw~zwoxz|fZD~@cy}Nbwxo{sqދ+ϋ{slfsssM^uɫxuwsqH>vDHRP~{|oǢՋ.'ES_`xEfqtxkp~r{E}%yfZWQ]szklZj{cd?*JW,Y>2NvVvVMVv_(X{{{{S]hwqrL_4X__%3}jvʠ˦&MJ%HJ%6OzCgVm_^tWzMms̋Lmslwvyv{z؋-Ѡv[j37S|}zPdNJɮ›H|HJvVN|WAt$SOvlXD5pk2Aoaዶ&lR:ijL~J\]lkd}^ijo<ŋ9vVv(VHV`viP˪tqiːƐs\Xl`IyrBYMygJOhrw|lr|q3}jv|OzCgVm_^tWzMms̋Lmslwvyv{z؋-3|H"4Mi(whW.Dqx¾ɋ͋?1Dgk}WudQ;xr%Z{kn}VXj^Ċٟļ3vVQTvv[K2Q1^|w͋5xWQ^ayTƬqz`8rssF|7Q[fndz>c{vΊ務ӥǘvVv&V.6IV6H|IIvVNVxRt͋',AMdg{'XUwj~BvQ"u YO]szkk~xcbQy׊ϋ TUv[K1G=GGb{[w^}r|w~l|}}T~G~nL܎n\$JDyQcqea{ыtmunr}}yyeUTB[iUdStQ6ލoix Sn}}vru]bOw.tŋ䑸;VVשᕩ]e]xċȋyY*u{||pRbϊUtf^vp\]GPRTcɊ܊U}r`~jvkaK9IhڠvvEVHEHVE]VrVv3/`vVvV/ )VvrBvV@VrrJ$*vvmv;idgQOVU[v{-v*VH;>/@]]oU{/^p`ˊ9 BVjktu{uYBy:5\ixKqrd ڠvvjJKKCLLF<DDN%:#\qy}aό]VQLmOtjvO3|H(MK%GJ%E"4Mi(whW.Dqx¾ɋ͋?1Dgk}WudQ;xr%Z{kn}VXj^ĊٟļvV`V~GͰPMJ%HJ%~nL܎n\$JDyQcqea{ыtmunr}}yyeUTB[iUdStQ6ލoix Sn}}vru]bOw.tŋ䑸vVvVG9D>C9EWVGH$CvvXGrxЋ☵ܤ¶Z}w}st0<$@+ Z_`d3v\y{{zz|Z|3v:hvn"hVbQ::7v`QWE|RYjWtzvyV*v VVs$ thVOtuulQ]ngPx]zm}cSKOaս{ŊrdCw)T`d}]{X}XÍclkn|ߋjtVKtKgxt|@YB;xƾfjhyrxy]^l)?vv\@&W#W;L[6s6s_DVV kwP.agm~@S-ڋquj|kH*v\ Nk}qH;3ֿɶd;cc}1WerovlQPuȊ멷̋owfcxs`d]yvswuzv{u|u}\lfuaeLSsT7]$vKVHc%A)Kz_^m:mEK0iJmvV>E2`~}}g;ac||o\nhQ}j{s^Q|fr|NjnY9=wyfmf_gvQG5_tËЅNhd|a FObt|~x{h|fq}NjŔǷ3v9BSP^^90F&u\N3ӼB83|sWotT;"û΋_nlu|ä‹[?ek|G^v{tWipOOΫstkkz`ys~[`D{Vj*C^du}`n]zIьvP3|Wp>.n:PG4'GvN)eЋˑꋲ1-YXou{wԐѩИ_jjy. <(+S[ky~o~d#K$ O<#<#3|=qBG;#%F8!_\laŠNlz}orvB燧̒勮]4agzyS[iz~|Kд)ȫQT:vjy}gSXLg{|uUcl|P)jyxw}xv{sVht\F#+37f`nhW@ 21g\qڋ싶k8]Zzcw Z{'߶ܐ&4`<:hH>vDHR)}y[Zt<]lrv4A l%Enwߋ}+r]EHt8Szm{\]m{9 Ȼ{ gae~}ghuIa3/Mc)gpƊ*ܭ2:*fP Gg8$oe7BoXYvqPPgvTۊ싨BЫNW v Mdsai~xWM%3v'HVjHvVvV@``;ok]vVvVDx{@Vv_v0}b b ~edC+t(C3vd"]$]3|X:mw~AqڋE͋Ë)LQ1g+#)ȥ²bh=Ve|oz]e+x+taIkp~(I>%Egym~Ы˩$ O;#8#Qxsq~`*[[^vkv`Xkz5J]X  endstream endobj 24 0 obj << /XHeight 915 /Ascent 905 /Type/FontDescriptor /StemV 64 /StemH 0 /CapHeight 706 /FontName/WLTLKJ+NeueHelveticaBQ-Light /Descent -191 /FontBBox[-166 -261 1042 971] /Flags 32 /ItalicAngle 0 /FontFile3 23 0 R >> endobj 25 0 obj << /FontDescriptor 24 0 R /Encoding 22 0 R /Name/F1 /Type/Font /Subtype/Type1 /FirstChar 32 /BaseFont/WLTLKJ+NeueHelveticaBQ-Light /Widths[292 250 0 0 0 833 0 0 250 250 354 562 208 375 208 354 562 562 562 562 562 562 562 562 562 562 250 250 0 562 0 0 0 646 688 708 688 604 542 750 729 229 0 667 562 854 708 750 646 750 688 625 562 688 604 896 583 0 604 0 0 0 0 500 0 521 583 521 583 521 271 562 562 208 208 500 208 854 562 562 583 583 333 479 292 562 458 729 479 458 479 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 521 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 562 0 0 0 0 562 0 354 0 0 0 0 0 542 833 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 688] /LastChar 208 >> endobj 26 0 obj << /BaseEncoding/MacRomanEncoding /Type/Encoding /Differences[120/x] >> endobj 27 0 obj << /Subtype/Type1C /Length 295 /Length1 295 >> stream GIXUIH+AGFigures+PiBQ>   ;bt(c) COPYRIGHT H. BERTHOLD AG 1993 Alle Rechte vorbehalten.Akzidenz-Grotesk Figures + PiAG Figures + Pi BQY,UW21XV13XW23VW32V  endstream endobj 28 0 obj << /XHeight 0 /Ascent 878 /Type/FontDescriptor /StemV 59 /StemH 0 /CapHeight 878 /FontName/GIXUIH+AGFigures+PiBQ /Descent -235 /FontBBox[-130 -235 1233 878] /Flags 34 /ItalicAngle 0 /FontFile3 27 0 R >> endobj 29 0 obj << /FontDescriptor 28 0 R /Encoding 26 0 R /Name/F2 /Type/Font /Subtype/Type1 /FirstChar 120 /BaseFont/GIXUIH+AGFigures+PiBQ /Widths[708] /LastChar 120 >> endobj 30 0 obj << /BaseEncoding/MacRomanEncoding /Type/Encoding /Differences[32/space] >> endobj 31 0 obj << /Subtype/Type1C /Length 271 /Length1 271 >> stream UOWHTB+NeueHelveticaBQ-Bold=Wk   ;Qb(c) COPYRIGHT H. BERTHOLD AG 1992 Alle Rechte vorbehalten.Neue Helvetica 75 BoldNeue Helvetica BQy6;T9  昞   endstream endobj 32 0 obj << /XHeight 901 /Ascent 891 /Type/FontDescriptor /StemV 138 /StemH 0 /CapHeight 706 /FontName/UOWHTB+NeueHelveticaBQ-Bold /Descent -182 /FontBBox[-195 -262 1034 983] /Flags 262178 /ItalicAngle 0 /FontFile3 31 0 R >> endobj 33 0 obj << /FontDescriptor 32 0 R /Encoding 30 0 R /Name/F3 /Type/Font /Subtype/Type1 /FirstChar 32 /BaseFont/UOWHTB+NeueHelveticaBQ-Bold /Widths[292] /LastChar 32 >> endobj 34 0 obj << /BaseEncoding/MacRomanEncoding /Type/Encoding /Differences[32/space] >> endobj 35 0 obj << /Subtype/Type1C /Length 268 /Length1 268 >> stream BUQOPL+NeueHelveticaBQ-Light=:_   ;Rc(c) COPYRIGHT H. BERTHOLD AG 1992 Alle Rechte vorbehalten.Neue Helvetica 45 LightNeue Helvetica BQz5J]X  endstream endobj 36 0 obj << /XHeight 915 /Ascent 905 /Type/FontDescriptor /StemV 64 /StemH 0 /CapHeight 706 /FontName/BUQOPL+NeueHelveticaBQ-Light /Descent -191 /FontBBox[-166 -261 1042 971] /Flags 34 /ItalicAngle 0 /FontFile3 35 0 R >> endobj 37 0 obj << /FontDescriptor 36 0 R /Encoding 34 0 R /Name/F4 /Type/Font /Subtype/Type1 /FirstChar 32 /BaseFont/BUQOPL+NeueHelveticaBQ-Light /Widths[292] /LastChar 32 >> endobj 38 0 obj << /BaseEncoding/MacRomanEncoding /Type/Encoding /Differences[32/space] >> endobj 39 0 obj << /Subtype/Type1C /Length 258 /Length1 258 >> stream OKKIJH+AGFigures+PiBQ>   ;bt(c) COPYRIGHT H. BERTHOLD AG 1993 Alle Rechte vorbehalten.Akzidenz-Grotesk Figures + PiAG Figures + Pi BQ  endstream endobj 40 0 obj << /XHeight 0 /Ascent 878 /Type/FontDescriptor /StemV 59 /StemH 0 /CapHeight 878 /FontName/OKKIJH+AGFigures+PiBQ /Descent -235 /FontBBox[-130 -235 1233 878] /Flags 34 /ItalicAngle 0 /FontFile3 39 0 R >> endobj 41 0 obj << /FontDescriptor 40 0 R /Encoding 38 0 R /Name/F5 /Type/Font /Subtype/Type1 /FirstChar 32 /BaseFont/OKKIJH+AGFigures+PiBQ /Widths[333] /LastChar 32 >> endobj 42 0 obj << /Type/Pages /Kids[43 0 R 44 0 R] /Count 2 >> endobj 43 0 obj << /Type/Page /Contents 8 0 R /Parent 42 0 R /Resources<>/ProcSet[/PDF/Text]/Font<>/ExtGState<>>> /MediaBox[0 0 566.92902 566.92902] >> endobj 44 0 obj << /Type/Page /Contents 16 0 R /Parent 42 0 R /Resources<>/ProcSet[/PDF/Text]/Font<>/ExtGState<>>> /MediaBox[0 0 566.92902 566.92902] >> endobj 45 0 obj << /Type/Catalog /Pages 42 0 R /PageLabels 17 0 R >> endobj 46 0 obj << /XPressPrivate(%%DocumentProcessColors: Black\n%%DocumentCustomColors: (PANTONE 7540 C)\n%%CMYKCustomColor: 0 0 0 .72 (PANTONE 7540 C)\n%%EndComments) /Producer(QuarkXPress(tm) 6.5) /CreationDate(20170111144934) /Creator(QuarkXPress(tm) 6.5) >> endobj xref 0 47 0000000000 65535 f 0000000015 00000 n 0000000059 00000 n 0000000111 00000 n 0000000157 00000 n 0000001872 00000 n 0000002414 00000 n 0000002460 00000 n 0000002507 00000 n 0000021412 00000 n 0000024091 00000 n 0000024638 00000 n 0000025792 00000 n 0000025929 00000 n 0000025996 00000 n 0000033381 00000 n 0000033926 00000 n 0000051991 00000 n 0000052030 00000 n 0000052397 00000 n 0000060179 00000 n 0000060411 00000 n 0000060999 00000 n 0000061463 00000 n 0000069873 00000 n 0000070101 00000 n 0000070792 00000 n 0000070880 00000 n 0000071256 00000 n 0000071475 00000 n 0000071646 00000 n 0000071737 00000 n 0000072089 00000 n 0000072321 00000 n 0000072496 00000 n 0000072587 00000 n 0000072936 00000 n 0000073164 00000 n 0000073340 00000 n 0000073431 00000 n 0000073770 00000 n 0000073989 00000 n 0000074158 00000 n 0000074222 00000 n 0000074500 00000 n 0000074761 00000 n 0000074830 00000 n trailer << /Size 47 /Root 45 0 R /Info 46 0 R /ID[<989832D307D03164360AFDF3FBAB4300><989832D307D03164360AFDF3FBAB4300>] >> startxref 75094 %%EOF