His Protein Pull Down Kit
產(chǎn)品名稱 | 規(guī)格 | 貨號(hào) | 目錄價(jià) |
His Protein Pull Down Kit | 5T | BK0055-t | 800 |
His Protein Pull Down Kit | 25T | BK0055-01 | 3000 |
產(chǎn)品介紹:
His Protein Pull Down Kit是一款能夠高效完成標(biāo)簽誘餌蛋白與靶蛋白共沉降的試劑盒。其包含高性能Co Smart Beads 6FF,能夠高特異性的捕獲帶有 6×、9×、12×His 標(biāo)簽的誘餌蛋白。另外試劑盒內(nèi)經(jīng)過(guò)優(yōu)化的緩沖液,為蛋白互作實(shí)驗(yàn)提供了最佳的反應(yīng)條件,增強(qiáng)了蛋白互作實(shí)驗(yàn)的穩(wěn)定性,更有效的去除實(shí)驗(yàn)背景。Co Smart Beads 6FF 是一種新型 IMAC 填料,螯合有非常牢固的Co 離子,擁有更為廣泛的適配性,試劑耐受情況見(jiàn)表1。
表1:Co Smart Beads 6FF試劑耐受情況
試劑種類 | 耐受時(shí)間 |
0.01M HCI, 0.01M NaOH | 1 week |
10mM EDTA, 1M NaOH, 5mM DTT, 5mM TCEP,20mM β-mercapyoethanol, 6M Gu-HCI | 24h |
500mM imidazole,100mM EDTA | 2h |
30% isopropanol | 20min |
本產(chǎn)品可廣泛應(yīng)用于大腸桿菌裂解物、細(xì)胞裂解物、細(xì)胞分泌上清等樣品中的蛋白互作驗(yàn)證,具體組分見(jiàn)表 2。
表2. His Protein Pull Down Kit產(chǎn)品組分
組分名稱 | 規(guī)格(5T) | 規(guī)格(25T) |
Co Smart Beads 6FF | 0.15ml | 0.75ml |
Lysis Buffer A | 5ml | 25ml |
Lysis Buffer B(1000x) | 0.1ml | 0.5ml |
Wash Buffer Enhanced | 2.5ml | 12.5ml |
Balance/Wash Buffer(10x) | 10ml | 50ml |
Elution Buffer | 0.4ml | 2ml |
Pull-down Column Accessory | 10套 | 50套 |